The Effect Of Exercise On Gut Microbiota And Sleep Quality Of Female College Students

Research purpose:Physical inactivity is a global public health problem,a key risk factor for chronic non-communicable diseases and the fourth independent risk factor for death worldwide.Gut microbiota is a complex microbial community,which has long-term interdependence with the host and is located in the gut of the host.Sleep and health are closely related,and it is one of the three health standards recognized by the international community.The purpose of this study is to understand the status quo of physical activity,intestinal flora and sleep quality of female college students by observing the changes of intestinal flora and sleep quality under the influence of exercise,this paper analyzes the“Dose-effect relationship”between exercise and body reaction from two aspects of intestinal microflora and sleep quality,so as to scientifically promote the improvement of physical activity level of female college students and establish an active sports life style,achieve a higher level of health.Research method:This study adopted a cross-sectional study design and recruited two groups of people from a university in Guangzhou.They were divided into an exercise group（female college students majoring in sports）and a less active group（non female college students majoring in sports）,each consisting of 15 people.The daily physical activity of female college students majoring in sports is mainly sports.Both groups used Acti Graph w GT3X-BT triaxial accelerometer and Bouchard physical activity diary to record daily average physical activity data for 7 days of wearing,and also wore the Firstbeat Bodyguard 2 fatigue recovery and exercise performance monitoring system to analyze their sleep quality.The obtained indicator data was statistically processed using SPSS 25.0 data statistics software,and descriptive statistics were expressed as mean±standard deviation（Mean±SD）,The mean difference between groups was compared using independent sample T-test or Wilcoxon rank sum test.The 16Sr RNA high-throughput sequencing technology was used on the Illumina Nova Seq sequencing platform to detect the fecal samples of the two groups of people.The data obtained were analyzed by variance analysis,rank sum test,alpha diversity analysis,PCA analysis,Anosim analysis,etc.The correlation between physical activity indicators and gut microbiota data,physical activity indicators and sleep indicators,and gut microbiota data and sleep indicators was analyzed using Pearson or Spearman correlation analysis.Research results:（1）The daily average energy consumption,metabolic equivalent,and daily moderate to high intensity physical activity（DM-VPA）in the exercise group were 323.99±140.01Kcal,1.32±0.08METs,50.90±24.79min,respectively,which were significantly higher than those in the low exercise group（178.01±69.11 Kcal,1.24±0.04METs,22.19±9.24min,P<0.05）.（2）There was no significant difference in Shannon index and Simpson index between the two groups（P<0.05）;The abundance of Fusobacterium（0.016±0.059）and Bacillus subtilis（0.00012±0.00001）in the less active group was higher than that in the exercise group（0.00017±0.00027）and Bacillus subtilis（0.00007±0.00003）（P<0.05）.（3）The sleep efficiency of the exercise group（95.79±1.84）was significantly higher than that of the less exercise group（90.59±4.22）（P<0.05）;The sleep index of the exercise group（66±0.05）was significantly higher than that of the less exercise group（44±0.13）（P<0.05）.（4）The abundance of Bacteroides（R=0.179,0.044,0.081,0.192）,Firmicutes（R=0.098,0.045,0.046,0.192）,Bifidobacteriaceae（R=0.115,0.019,0.066,0.047）,and Lactobacillus（R=0.300,0.282,0.234,0.031）was positively correlated with sleep efficiency,sleep index,sleep duration,and recovery index（P<0.05）,The abundance of Bacillus thuringiensis(R=-0.489^**,-0.470^**,-0.440^**,-0.095)and Fusobacterium（R=0.129,-0.010,-0.109,-0.317）was negatively correlated with sleep indicators（P<0.05）.Research conclusion:（1）The species diversity and evenness of intestinal microflora in the two groups were similar,but the species richness of intestinal microflora in the exercise group was higher than that in the hypokinetic group;Exercise can increase the amount of beneficial bacteria（bacteroidetes,verruciales,bifidobacterial,Lactobacillus,Pratocor）,inhibit the formation of harmful bacteria（bacillus,Clostridium）.（2）The sleep quality of the exercise group was better than that of the control group,moderate exercise can improve the quality of sleep.（3）The abundances of Bacteroidetes,verrucae,Bifidobacteriaceae and Lactobacillus were positively correlated with sleep quality,while the abundances of bacillus and Clostridium were negatively correlated with sleep quality Lack of exercise can lead to increased levels of Clostridium,bacillus,and bacteroidetes,Verruciaceae,Bifidobacterium,Lactobacillus,and decreased levels of sleep quality.