Effects Of MICT On Plasma Levels Of Gal-3,sST2,PTX3,YKL-40,and Nexilin In College Students

Objective:Exercise training can promote cardiovascular health.Among various training programs,moderate-intensity continuous training（MICT）is considered an exercise regimen that promotes cardiovascular health.Studies have shown that plasma biomarkers such as Gal-3,sST2,PTX3,YKL-40,and Nexilin,which are associated with cardiovascular diseases,exhibit changes in concentration.However,there is still limited research on the relationship between these cardiovascular biomarkers and MICT.Therefore,this study aimed to investigate the effects of MICT on the concentrations of Gal-3,sST2,PTX3,YKL-40,and Nexilin in plasma by intervening with MICT in healthy male university students.The study aimed to explore the impact of MICT on these biomarkers and investigate the potential reasons for their changes.Methods:The study included 11 healthy male college students with no regular training experience.They underwent six weeks of MICT,with running training three times a week.The MICT protocol consisted of running at 60%VO2peak for 30 minutes per session.Samples were collected at different time points:before and after the six weeks of MICT for cardiac ultrasound and cardiopulmonary exercise testing,and before and after the six weeks of MICT as well as four weeks after cessation of training for peripheral blood samples at rest.Additionally,another peripheral blood sample was taken at rest before and immediately after acute MICT exercise at the end of the training.The concentrations of NT-pro BNP,hs-c Tn T,Gal-3,sST2,PTX3,YKL-40,and Nexilin in plasma under different conditions were measured using the ELISA method.Paired sample t-tests were used to compare the indicators before and after acute MICT and before and after the six weeks of MICT,and repeated measures analysis of variance was used to analyze the indicators at the six-week MICT time point and four weeks after cessation of training.Results:（1）The effects of six weeks of moderate-intensity continuous training（MICT）on cardiopulmonary function in the subjects:After six weeks of MICT,the LVEF was higher than before six weeks of MICT（P<0.05）.Additionally,AT,VO_2AT,VO_2AT/HR_AT,VE_AT,and OUPES were also higher than before six weeks of MICT（P<0.01）;（2）Plasma concentrations of Gal-3 and PTX3 were higher after acute MICT compared to before acute MICT（P<0.05）.Additionally,plasma concentrations of NT-pro BNP,hs-c Tn T,sST2,YKL-40,and Nexilin were also higher after acute MICT compared to before acute MICT（P<0.01）;（3）After six weeks of MICT,the plasma concentrations of NT-pro BNP and Gal-3 during rest were higher than before six weeks of MICT,showing significant differences（P<0.05）.The plasma concentrations of hs-c Tn T,sST2,PTX3,YKL-40,and Nexilin during rest were also higher than before six weeks of MICT,showing extremely significant differences（P<0.01）.After four weeks of cessation of training,the plasma concentration of NT-pro BNP during rest was lower than after six weeks of MICT（P<0.05）,and the concentrations of hs-c Tn T,Gal-3,sST2,PTX3,YKL-40,and Nexilin were also lower than after six weeks of MICT,showing extremely significant differences（P<0.01）.After four weeks of cessation of training,there were no significant differences in plasma concentrations of all indicators compared to the concentrations during rest before six weeks of MICT（P>0.05）.Conclusion:Acute MICT can increase the levels of Gal-3,sST2,PTX3,YKL-40,and Nexilin in plasma in male university students.After six weeks of MICT,an increase in the levels of relevant cardiac biomarkers during rest was observed,but the plasma concentrations remained within the normal range.After four weeks of detraining,these changes can return to the resting levels before exercise training.