Development Of Seawater Algae Fluorescence In-situ Sensor

Marine phytoplankton is the basis of marine primary productivity,and its content,distribution,and temporal and spatial dynamic changes are the basis for the study of regional marine eutrophication levels,biogeochemical cycles and other scientific issues.In particular,as red tides and green tides in my country's coastal areas have become normal in recent years,acquiring long-term,real-time,and continuous data on marine phytoplankton and understanding the distribution and changes of phytoplankton multitemporal and spatial dynamics has become an urgent task for marine business workers.In the current marine ecological environment monitoring technology system,chlorophyll and blue-green algae are important monitoring indicators for retrieving phytoplankton biomass in water bodies,and are key elements for monitoring and warning and forecasting marine red tide and green tide disasters.Development of seawater chlorophyll and blue-green The algae in-situ sensor is very necessary to realize the long-term,in-situ,real-time and continuous monitoring of seawater chlorophyll and blue-green algae.Based on the comprehensive analysis of the existing technology of chlorophyll and blue-green algae monitoring at home and abroad,this thesis uses digital lock-in amplification technology to focus on solving the technical problems of weak fluorescence signals of chlorophyll a and phycocyanin in seawater and interference by ambient light..In addition,based on this technology,comprehensive use of marine chemical machinery,electronic information,optics and other knowledge to design the optical path,circuit,and mechanical mechanism of the sensor,completed the development of chlorophyll and blue-green algae in-situ sensors,and carried out related performance tests?Related performance verification and sea trials show that the detection limit of the developed chlorophyll sensor is 0.05 ?g/L,the detection linear range is 0?200 ?g/L,the precision is better than than 1 %,and it can be continuously and stably in seawater for a long time.run.The comparison test with the Japanese Aggreko AAQ171 multiparameter sensor proves that the stability and reliability of the sensor is close to similar foreign products;the detection limit of the blue-green algae in-situ sensor is 0.05 ?g/L,and the detection linear range is 0?100 ?g/L,the precision is better than 2%.The abovementioned sensor technology is autonomous and controllable,and can be widely used in marine ecological monitoring platforms such as marine buoys and stations.