| Hyperlipidemia is a high risk factor for cardiovascular disease.Prevention and improvement of blood lipid status is of great significance for the prevention and treatment of cardiovascular disease.At present.there are many clinical lipid-lowering drugs.Although the short-term lipid-lowering effect is good,some drugs are easy to cause side effects such as liver injury,muscle pain,cognitive impairment risk and so on.The concept of "blood lipid" in modern medicine is similar to that of "ointment lipid" in Huangdi Neijing.Ointment lipid originally belongs to the subtle nutrients derived from the valley.When its metabolism is abnormal,its transportation and distribution are not timely,the formation of blood components increase,blood vessel is dirty,blood flow is slow,gradually accumulated.and phlegm and blood stasis are produced.In this way,the process of Valley turbid lipid phlegm and blood stasis is formed from physiology to pathology.Just as hyperlipidemia is a risk factor for atherosclerosis,this state of blood vessel turbidity is the early stage of the formation of tangible phlegm and blood stasis.The smooth passage of blood veins needs the promotion and warmth of qi,and the subtle distribution of plaster fat needs the transpiration and gasification of spleen and kidney qi.Therefore,in the treatment of hyperlipidemia with turbid blood veins,while eliminating phlegm and blood stasis and smoothing blood veins,we should invigorate Qi and strengthen the integrity,taking into account the deficiency of spleen and kidney.On the basis of the above understanding,the study group used self-designed Buqi and turbid powder(Sheng Huangqi,Jiu Dahuang,Sheng Shan)in clinical practice.Hawthorn et al.)has a good effect on hyperlipidemia.In this experiment,the hyperlipidemia rat model was used to test its lipid-lowering effect and explore the possible mechanism of action,in order to provide experimental data support for further promotion of this method.1 ObjectiveTo observe the effect of Tonifying Qi and resolving turbidity Powder on hyperlipidemia in rats,and to explore its pharmacological effects.2 MethondsSixty male SD rats of SPF grade were randomly divided into normal control group(10 rats),high-fat model group(50 rats)and normal control group(50 rats).Rats in model group were fed with high-fat diet for four weeks to establish hyperlipidemia rat model.After successful modeling,rats in model group were divided into normal control group,high-fat model control group and Qihuang Laihao San low.medium and high dosage groups.Quantity group.After successful modeling,the high fat model control group and the three drug groups were still fed with high fat diet.At the same time,the three drug groups were given intervention.The dosage of intragastric administration was 0.4575 g/kg*d in the low dose group.0.915 g/kg*d in the middle dose group and 1.83 g/kg*d in the high dose group.The normal control group and the hyperlipidemia model control group were given 10 ml/kg*d distilled water by gavage.After 5 weeks of drug intervention.the general situation of rats was observed.The weight of rats was recorded once a week.At the 5th weekend,the serum total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C),superoxide dismutase(SOD),hydroxymethylglutarate monoacyl coenzyme A reductase(HMG-CoA reductase)were measured.Activity,whole blood viscosity,cut liver tissue,make liver tissue slices,detect apolipoprotein A5(Apoa5)protein expression in liver tissue,and cholesterol regulated SREBP-2 gene expression level.3 Results3.1 Liver indexCompared with the blank group,the liver index of rats in the high-fat model group increased significantly,P<0.05,the difference was statistically significant,the liver coefficient of rats in the three drug groups decreased,compared with the high-fat model group,P<0.05,the difference was statistically significant.3.2 Changes of blood lipidsFour weeks after feeding with high-fat diet,compared with the blank group,the serum total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C)in the high-fat model group increased,P<0.05,the difference was statistically significant;the high density lipoprotein cholesterol(HDL-C)decreased,but P>0.05,the difference was not statistically significant.The hyperlipidemia rat model was successfully established.After 5 weeks of drug intervention,compared with the blank group,the serum total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C)in the high-fat model group were still increased and high density lipoprotein cholesterol(HDL-C)was decreased.P<0.05.Compared with the high-fat model group.the serum total cholesterol(TC).triglyceride(TG)and low density lipoprotein cholesterol(LDL-C)in the low,middle and high drug groups decreased significantly(P<0.01),and the high density lipoprotein cholesterol(HDL-C)increased significantly(P<0.01).3.3 Changes of Enzyme Activities Related to Lipid Metabolism3.3.1 Changes of serum HMG-CoA reductase activity in ratsThe activity of HMG-CoA reductase in serum of rats in high-fat model group was 33.712.10 U/ml,compared with 21.292.65 U/ml in blank group.P<0.05,the difference was significant,and the activity of HMG-CoA reductase in serum of rats in high-fat model group increased.The activity of serum HMG-CoA reductase in the low,middle and high drug groups was 23.662.42 U/ml.22.013.03 U/ml and 21.522.19 U/ml,respectively.There was no significant difference between them(P>0.05),but compared with the high-fat model group,the activity of serum HMG-CoA reductase in the three drug groups was different and decreased.3.3.2 Changes of superoxide dismutase(SOD)activityThe SOD activity of rats in high fat model group was 125.145.32 U/ml,compared with 59.147.83 U/ml in blank group,P<0.05,the difference was significant,and the SOD activity of rats in high fat model group was decreased.The activity of SOD in the low,middle and high dose groups were 123.527.97 U/ml,133.487.01 U/ml and 145.729.19 U/ml.respectively.Compared with the high fat model group,the activity of SOD in the middle and high dose groups was significantly higher than that in the low dose group(P>0.05).3.4 Changes in blood viscosityCompared with the blank group,the whole blood viscosity,plasma viscosity,hematocrit,erythrocyte sedimentation rate,erythrocyte rigidity index and erythrocyte aggregation index in the high-fat model group increased significantly(P<0.05).Compared with the high-fat model group,the low shear,middle shear,high shear whole blood viscosity,plasma viscosity,hematocrit.erythrocyte sedimentation rate,erythrocyte rigidity index and erythrocyte aggregation index of the rats in the middle and high dose groups decreased(P<0.05);the low shear,medium shear,high shear whole blood viscosity,plasma viscosity,hematocrit and erythrocyte sedimentation rate of the rats in the low dose group decreased(P<0.05),erythrocyte rigidity index decreased(P<0.05).The erythrocyte aggregation index also decreased.P>0.05,there was no significant difference.3.5 Changes of pathological sections of liver tissueThe liver tissue of the blank group was pale red and glossy.Under light microscopy,the hepatic lobules were clearly visible,and the hepatocytes were in normal shape and arranged into hepatic cords.The liver tissue of rats in the high-fat model group was pale,swollen and dark.Under light microscope,there were vacuoles formed by fat droplets in the liver cells.showing diffuse steatosis.The fatty degeneration of liver tissue and lipid droplets in hepatocytes were reduced in the low.middle and high drug groups.3.6 Changes of apolipoprotein A5(Apoa5)gene expression in liver tissueCompared with the blank group.Apoa5 gene expression in liver tissue of rats in the high-fat model group was significantly down-regulated.P<0.05,the difference was statistically significant.Compared with the high-fat model group,Apoa5 gene expression was up-regulated in the liver tissues of rats in the low,middle and high drug groups,P>0.05.and there was no significant difference among the three drug groups.3.7 Changes of Cholesterol Regulating Element 2 Protein(SREBP-2)in Liver TissueCompared with the blank group,the expression of SREBP-2 protein in liver tissue of rats in the high-fat model group was down-regulated,P>0.05,and the difference was statistically significant.Compared with the hyperlipidemia model,the expression of SREBP-2 protein in liver tissue of rats with hyperlipidemia was up-regulated in the low,middle and high dose groups(P<0.05).4 ConculsionTonifying Qi and resolving turbidity Powder can reduce serum total cholesterol(TC),triglyceride(TG)and low density lipoprotein cholesterol(LDL-C)in hyperlipidemia rats induced by high-fat diet to regulate blood lipids.It can also improve blood viscosity,enhance SOD activity and reduce the damage caused by lipid peroxidation in hyperlipidemia.The mechanism of its lipid-lowering effect may be related to the inhibition of HMG-CoA reductase activity,the up-regulation of APoa5 gene and the accelerated metabolism of TC and TG by SREBP-2 protein. |
PDF Full Text Request