| Objective: By observing the apoptosis index,sirt1,SIRT1 / Runx2 pathway and SIRT1 / PPAR? pathway in rat Achilles tendinopathy,to explore the mechanism of Achilles tendinopathy and observe the the treatment of the Zheng's NO.1 new wound medicine and provide a theoretical basis for the clinical use.Method: 72 male Wistar rats were randomly divided into K group(blank control group),T group(pure jump group)and F group(pill jumping group),and each group was randomly divided into 4 weeks group according to the time of collection.6 Week group,8 weeks group,8 in each group.The rats in group K were naturally fed,group T and group F were modeled by "shock-jump method",and group F was treated with fresh wounded Zheng No.1 from the 4th week.After observing the general condition of the rats(hair,air state,physical condition,etc.)and the number of effective jumps,Achilles tendons were taken at the 4th,6th,and 8th weekends respectively.HE staining was used to observe the histomorphological changes.Tunel method was used to detect the apoptosis index.The mRNA and protein expression of deacetylase sirt1,transcription regulators Runx2 and PPAR? were detected by PCR and Western Bolt.Results: 1.General observations: Gross observation: The rats in group K were generally in good condition.After jumping in the model group(T group,F group),the rats exhibited awful tiredness,disorganized fur,and decreased physical strength,but the F group was superior to the T group.2.The number of effective jumps: As a whole,the model group(T group,F group)showed a downward trend during the jump,and the F group was superior to the T group,and there was a significant difference in the 4 to 7 weeks.3.Histomorphological observation of HE staining: In the K,4,6 and 8 weeks,the iliac crest fibers were arranged in a wavy form with no significant difference.4.Apoptosis test:(1)Intragroup analysis: The K group and F group showed a downward trend from the 4th week to the 8th week,and the T group showed a slowly rising trend.There was no significant difference between group K and group T,P>0.05.There was significant difference between week 4 and week 8 in group F,P<0.05.(2)Between-group analysis: At the 4th week,there was significant difference between group K and group F and group T,P<0.05;K,T group at week 6,group K,group F and group T at 8 weeks.Significant difference occurred,P<0.05.Except for 4 weeks,the apoptosis index of group T and group F were basically the same but significantly higher than that of group K.At week 6 and 8 T was significantly higher than that of group K and F.5.Expression of sirt1:(1)mRNA: Analysis within 1 group: At 4 to 8 weeks,the T group first decreased and then increased.There was a significant difference between week 6 and week 8 in group T and group F,P<0.05.Analysis between two groups: There was no significant difference among the three groups,P>0.05.(2)Protein expression: Intragroup 1 analysis: At 4 to 8 weeks,T group first decreased and then increased.There was no significant difference among the three groups(P>0.05).Between the two groups,there was a significant difference between the K and T groups at week 8,P<0.05.At week 4,6,8 weeks,T group was higher than F group and K group.6.Expression of PPAR?:(1)mRNA: Analysis within one group: At 4 to 8 weeks,T showed an overall increase,and K and F groups maintained a basically constant trend.There was a significant difference between 4 weeks and 8 weeks in group T,P<0.05.There was no significant difference between K and F groups,P>0.05.Analysis between two groups: At the 4th week,there was a significant difference between group T and group F,P<0.05.At week 6,there was a significant difference between group K and group T,P<0.05.At the 8th week,there was significant difference between K,F group and T group,P<0.05,and T group was higher than K,F group at 4,6 and 8 weeks.(2)Protein expression: Within the first group analysis,T showed an overall upward trend at 4 to 8 weeks,while K and F groups showed no significant change.There was no significant difference among the three groups,P>0.05.Analysis between two groups: At the 8th week,there was significant difference between K,F group and T group,P<0.05.At the 4th,6th,and 8th weeks,the expression of the three groups was highest in the T group,followed by the F group,and lowest in the K group.7.Runx2 expression(1)mRNA: Analysis within 1 group: There was a significant difference between week 6 and week 8 in group T,P<0.05.Between the two groups: 6 and 8 weeks,there was a significant difference between the K and T groups.At 4,6,and 8 weeks,the T group was the highest among the three groups.(2)Protein expression: Within group 1 analysis: During 4-8 weeks,T showed an overall upward trend,and there was no significant difference among K,T and F groups(P>0.05).Analysis between two groups: At the 8th week,there was significant difference between K,F group and T group,P<0.05,and at the 4th,6th,and 8th weeks,the expression of the three groups was the highest in the T group.Conclusion: 1.Apoptosis of tendon cells can affect the process of tendinopathy.As the Achilles tendons apoptosis cells increases,the tendinopathy be more serious.2.The expression of sirt1 can be detected during the formation of tendinopathy,and through the Sirt1/Runx2 pathway influences the ossification process,and Sirt1/PPAR? influences the process of fatification.3.The external application of Zheng's No.1 new wound medicine can effectively delay the development of tendinopathy,whose mechanism has a great relationship with the Inhibition of Apoptosis,reduction the expression of Runx2,to inhibit ossification and reduce the expression of PPAR? to inhibit fatification... |
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