Study On Physical And Chemical Factors Related To Pathology And Physiological Ossification Of Tendon

The tendon is the mechanical tissue that connects the bones and muscles.Tendon-related diseases,such as tendon tear and tendinopathy,seriously impact on people's normal work and life.But tendon treatment is aways unsatisfactory,the main reason is the lack of awareness of the pathogenesis of tendon disease.Stem cells are chemically or physically induced to differentiate into bone,tendon,adipose and cartilage.It is a good platform for studying cell differentiation and is an ideal seed cell for tissue regeneration.During the repair of the tendon,stem cells play an important role in tendon disease.But some of the stem cells in the repair process will be into the chondrogenesis and osteogenesis differentiation,lead to the disease further development.In the ligament-bone reconstruction,we hope that stem cells to chondrogenesis and osteogenesis in order to promote ligament-bone repair.Therefore,control of stem cell abnormal differentiation is the focus of treatment of tendon-related diseases.Preliminary results indicate that Racl has a significant activity in tendon disease.And the topology of the material has an effect on stem cell differentiation.In this study,tendon stem/progenitor cells(TSPCs)and bone marrow mesenchymal stem cells(BMSCs)were used to study the method of treating tendon-related diseases by controlling the differentiation of stem cells.The regulation of stem cell differentiation was observed by the use of small molecules to inhibit Racl activity and the preparation of composite sequence biomimetic scaffolds.And its effect on the repair of tendon-related diseases.In vitro study,NSC23766 inhibition of Racl activity can effectively inhibit tendon degeneration and calcification related gene expression.The calcification of TSPCs was also significantly inhibited.The use of NSC23766 in the tendinopathy model can significantly reduce Racl activity in TSPCs.While inhibiting the degradation of the tendon matrix,reducing the formation of calcification.And the random decellerated tendon matrix induced BMSCs chondrogenesis and osteogenesis differentiation.In the rabbit anterior cruciate ligament(ACL)reconstruction model,the random-aline-random scaffold can significantly enhance the ability of bone and fibrous cartilage formation,more effective repair tendon-bone connection.Indicating that inhibition of related genes and changes in material topology can be effective in controlling stem cell differentiation,help tendon-related diseases,treatment and repair.