Effects Of Lian Chen Decoction On Body Weight And Serum Inflammatory Factors In Obese Mice

Objective Objective to establish the obesity model of C57 BL / 6J mice induced by high-fat diet.The intervention effect of Lian chen Decoction on the weight and morphological changes of epididymis adipose tissue and liver,serum lipid metabolism and inflammatory factor levels were observed to explore the mechanism of Lian chen Decoction in reducing the weight of obese mice.Method Fifty C57 BL / 6J mice were randomly divided into two groups: the blank group was fed with normal diet(n = 10),and the model group was fed with high-fat diet(n = 40).At the end of the 16 th week,the body weight of the mice was measured.The success standard of the model was that the weight of the model group was more than 20% of the weight of the blank group.Thirty mice were randomly divided into three groups: model group,simvastatin group and Lian chen Decoction group,with 10 mice in each group.The administration group was given simvastatin 4.5mg/kg/d and Lian chen Decoction7.47g/kg/d respectively.The blank group was given ordinary feed,while the model group,simvastatin group and Lian chen Decoction group were given high-fat diet.The weight of mice was measured weekly.The mice were fasted for 12 hours after the last administration and weighed.Mice were anesthetized with 4% chloral hydrate solution,and blood was collected from eyeballs.The serum levels of TG,TC,LDL-C and HDL-C were detected by automatic biochemical analyzer.Serum levels of TNF-?,IL-6,IL-1 ?,CRP and MCP-1 were detected by ELISA.The adipose tissue and liver tissue of epididymis were dried with filter paper and weighed.Some tissues were fixed in 4% paraformaldehyde fixative for HE staining.Result 1.After 16 weeks of high-fat diet,the weight of mice in model group was significantly higher than that in blank group fed with normal diet(P < 0.01),and the weight of model group was more than 20% of that of blank group,indicating that the establishment of obesity model was successful.Compared with the model group,the weight of mice in Lian chen Decoction group decreased significantly(P < 0.01).The results showed that Lian chen decoction could reduce the weight of obese mice induced by high-fat diet.2.HE staining of epididymal adipose tissue and liver showed that there were a large number of lipid droplets accumulation in the epididymis adipose tissue and liver cells in the model group;meanwhile,the weight of epididymal adipose tissue and liver was significantly higher than that of the blank group(P < 0.01).The lipid droplets in adipose tissue and liver cells of mice in Lian chen Decoction group were significantly reduced,and the weight of epididymis adipose tissue and liver was significantly lower than that of model group(P < 0.01).The results showed that Lian chen decoction could reduce the fat accumulation in adipose tissue and liver.3.Compared with the blank group,the contents of TG,TC and LDL-C in the model group were significantly increased(P < 0.01),and the HDL-C level was significantly decreased(P < 0.05).Compared with the model group,the contents of TG and LDL-C in serum of Lian chen Decoction group were significantly decreased(P < 0.01),TC content was significantly decreased(P < 0.05),and HDL-C level was significantly increased(P < 0.05).The results showed that Lian chen decoction could regulate blood lipid metabolism and improve dyslipidemia in obese mice.4.The serum levels of TNF-?,IL-6,CRP,IL-1 ? and MCP-1 in the model group were significantly higher than those in the blank group(P < 0.01).The levels of TNF-?,IL-6,IL-1 ? and MCP-1 in Lian chen Decoction group were significantly lower than those in model group(P < 0.01),and CRP levels were significantly lower(P < 0.05).The results showed that Lianchen decoction could significantly reduce the level of serum inflammatory factors and improve the systemic inflammatory response in obese mice.Conclusion 1.Lian chen decoction can significantly reduce the weight of obese mice and achieve the purpose of treating obesity.2.Lian chen decoction can regulate blood lipid level,reduce fat accumulation and improve abnormal lipid metabolism.3.Lian chen decoction can reduce the level of serum inflammatory factors in obese mice,which may be the potential mechanism of Lian chen Decoction in treating obesity.