The Experimental Study On The Effect Of Yunnan Spring-dryness Environment On Airway Fluid Metabolism In Mice

Objective:Based on the previous foundation of research,followed by developing animal models with Yunnan spring-dryness syndromes.Guided by the theory of Traditional Chinese Medicine Etiology,applying Yunnan spring-dryness environment to mice.And observe its general biological characterization,weasand,structure of lung tissue and contents of RS?AQP5?DPPC?IL-8 in airway.And "assessing syndromes through therapeutic effect of prescription",discussion on the effect of Yunnan spring-dryness environment on airway body fluid metabolism in mice.Analyze the internal correlation between the above indicators and "excessive dryness leads to fluid loss","dryness invades the defenses of the lungs"," the dispersing mechanism of the defenses of the lungs is delayed"," loss of lung fluid due to desiccation"," Wei Qi resists the invasion of exogenous pathogens" in Chinese medicine.In order to objectively evaluate the scientificity,rationality and reliability of animal models from multiple levels such as macroscopic appearance,organizational structure,and microscopic molecules,the aim is to provide a basis for exploring a scientific,reasonable,and reproducible animal model of Yunnan Spring Dryness Syndrome.Methods:Randomly divide 60 Kunming mice into 4 groups,normal group,model group,water addition group,Sang Xing decoction,15 mice in each group,all fed into artificial climate incubator.The normal group is configured for normal temperature and humidity environment,fed with conventional food;the three groups of the model group,the water-added group,and the Sang Xing decoction are all configured for the Yunnan spring-dryness environment,and fed with spicy and dry food;From the first day of the intervention element,gavage treatment will be given at 8 o'clock every day.Sang Xingdecoction group will be given Sang Xing decoction,and water group will be given boiled water for 14 days.During the experiment,the mice were observed daily for changes in general characteristics,diet,drinking water,body mass,etc.,and materials data will be obtained on the 15 th day.Observe and detect the weasand,pathogeny structure of lung tissue and contents of RS?AQP5?DPPC?IL-8 in airway of experimental mice.Results:1 Effect of Yunnan spring-dryness environment on general biological characterization of miceNormal mice in the normal group were naturally active and mentally well during the experimental feeding period.The nails were shiny and lustrous,with smooth hair,clear urine,soft waste,few tears and no scratches.Compared with the normal group,the model group gradually showed anxiety and irritability after the intervention condition was applied,the nails were dark and dry,the fur was wrinkled and curled,the shedding was heavy,the urine was yellow,and the waste was hard and dry.The signs of tears and bite are frequent and obvious,blood spots on the back of the tail are could be seen;the water-added group is slightly better than the model group,and the mood is also irritable,the nails are dark and dry,the fur is dry and frizzy,the urine is yellow,waste is slightly dry,a few tearing,and a small amount of scratches on the tail and back;Sang Xing decoction group occasionally had abnormal emotions at first,and later recovered,and his consciousness was clear,the color of the nails tended to be normal,and the hair was shiny and slightly rough,urine is slightly yellowish,the waste is slightly moist,few tears,and few traces on the back of the tail.Compared with the normal group,the water consumption of the experimental mice in the model group increased(P> 0.05),and the dietary intake decreased significantly(P<0.01);the water consumption in the water-added group and the Sang Xing decoction group increased slightly(P > 0.05),the dietary amount slightly decreased(P> 0.05).Compared with the model group,the water consumption of the experimental mice in the water-added group was relatively decreased(P> 0.05),and the dietary amount was relatively increased(P> 0.05);The amount increased significantly(P <0.05).2 Effect of Yunnan spring-dryness environment on structure and morphology of trachea and lung in miceIn the normal group of experimental mice,the tracheal cavity morphology and structure were clear and regular,and the surface of the mucosa layer was neat,without damage and congestion;the lung tissue structure and morphology were clear and complete,and the alveolar wall structure was intact with thin vacuoles.The experimental mice in the model group,the tracheal mucosa showed obvious local damage and thinning,partial ciliary skin cells were partially damaged and broken,a large number of inflammatory cells in the mucosa layer,partly infiltrated the transparent cartilage layer,and blood vessel walls were congested;The airway remodeling of lung tissue is slightly deformed,the bronchial wall has slightly hyperplasia,the bronchiolar part is broken off and the wall is thickened with the formation of inflammatory follicles,the inflammatory cells infiltrate the muscle layer,a large number of red blood cells and viscous liquid exudate,alveolar fusion,obvious congestion can be seen in the tube.The experimental mice in the water-added group,the tracheal mucosa showed some hyperplasia with inflammatory cells,cilia epithelial cells were partially shed,and inflammatory cells infiltrated.The lung bronchial muscular is hyperplastic and thickening,there are a large number of inflammatory cells and erythrocytes,the alveolar wall partly disappeared and merged into pulmonary bullae,and the inflammatory cells infiltrated.The experimental mice in the Sang Xing decoction group had regular tracheal morphology and structure,complete mucosal layer,few inflammatory cells,and few ciliated columnar epithelium shed or missing;the bronchial structure of lung tissue was clear,there was no bleeding in the cavity,and the alveolar wall was slightly exude,bleed,and there are a small amount of inflammatory cells.3 Effects of Yunnan spring-dryness environment on the contents of RS? AQP5? DPPC and IL-8 in the Airway of mice3.1 Effect on RS content in mice airwayCompared with the normal group,the airway RS content of the experimental mice in the model group was significantly reduced(P <0.01);the airway RS content of the experimental mice in the water group was significantly reduced(P <0.05).Compared withthe model group,the airway RS content of the experimental mice in the water-added group decreased(P> 0.05);the airway RS content of the experimental mice in the Sang Xing decoction group increased significantly(P <0.01).3.2 Effect on AQP5 content in mice airwayCompared with the normal group,the airway AQP5 content of the experimental mice in the model group and the water group was significantly reduced(P <0.05);the airway AQP5 content of the experimental mice in the Sang Xing decoction group was slightly reduced(P> 0.05).Compared with the model group,the airway AQP5 content of the experimental mice in the water-added group increased slightly(P> 0.05);the airway AQP5 content of the experimental mice in the Sang Xing decoction group increased significantly(P <0.05).3.3 Effect on DPPC content in mouse airwayCompared with the normal group,the airway DPPC content of the experimental mice in the model group decreased significantly(P <0.01);the airway DPPC content of the experimental mice in the water group decreased significantly(P <0.05);the airway DPPC content of the experimental mice in the Sang Xing decoction group slightly reduced(P>0.05).Compared with the model group,the airway DPPC content of the experimental mice in the water group increased slightly(P> 0.05);the airway DPPC content of the experimental mice in the Sang Xing decoction group increased significantly(P <0.05).3.4 Effect on IL-8 content in mouse airwayCompared with the normal group,the IL-8 content in the airway of the experimental mice in the model group and the water group increased significantly(P <0.05);the airway IL-8 content of the experimental mice in the Sang Xing decoction group increased slightly(P> 0.05).Compared with the model group,the airway IL-8 content of the experimental mice in the water-added group was slightly reduced(P> 0.05);the airway IL-8 content of the experimental mice in the Sang Xing decoction group was greatly reduced(P> 0.05).Conclusions:(1)Under the Yunnan spring-dryness environment,the general biological characterization of mice showed the sign of dryness and Shaojin,according to theprinciple of "Siwaichuainei" in Traditional Chinese Medicine and the theories of "dryness and dryness,chapping,all of which belong to dryness" and "Excessive dryness leads to fluid loss",it indicated that there was dryness syndrome in mice,thus showing the sign of dryness syndrome.The macroscopic characterization of mice conformed to the basic characteristics of animal models of dryness syndrome.And the morphology of trachea and lung tissues of mice showed different degrees of injury and changes in the contents of RS?AQP5? DPPC and IL-8 in the airways,further indicating that the Yunnan spring-dryness environment has a certain degree of effect on the physiological function,organ tissue structure,and airway micromolecules of mice?(2)The contents of RS,AQP5,DPPC and IL-8 in the airways of mice were changed by Yunnan spring-dryness environment,indicating that the body fluid of mice in the environment of spring dryness in Yunnan Province was damaged,which affected the function of lung promoting Wei Qi and defense against evil,and the immune response occurred in the airways.From the microscopic level,the effects of spring-dryness environment on the function of lung controlling Wei,transporting body fluid and lung protecting evil were explained.(3)According to the corresponding principle of "there is syndrome with the corresponding formula",applying the method of "syndrome differentiation through formula effect assessment",after the intervention of Sangxing Decoction,a prescription for treating dryness,the characterization of dryness syndrome,histological examination and airway RS? AQP5? DPPC? and IL-8 of mice were improved to different extents,indicating that this experimental animal model has the attributes of Yunnan spring dryness syndrome.(4)Under the Yunnan spring-dryness environment,the contents of RS,AQP5 and DPPC in the airways of mice were significantly reduced,and the content of IL-8 increased significantly.After the intervention of Sangxing Decoction,the contents of RS,AQP5 and DPPC in the airways of mice were increased,and the content of IL-8 decreased.These results indicate that the spring dryness environment in Yunnan can have an effect on both airway body fluid metabolism and lung health function in mice,which is consistent withthe theories of "excessive dryness leads to fluid loss" ? "dryness invades the defenses of the lungs" ? " the dispersing mechanism of the defenses of the lungs is delayed" ? " loss of lung fluid due to desiccation" ? " Wei Qi resists the invasion of exogenous pathogens" in traditional Chinese medicine.RS,AQP5,DPPC and IL-8 were preliminarily included in the observation and evaluation index system of animal model of Yunnan Spring Dryness Syndrome for further study.(5)The indexes of mice in Sangxing decoction group were significantly better than those in water group,indicating that the characterization of dryness syndrome caused by the Yunnan spring-dryness environment was due to the damage of body fluid by wind and dryness evil,the evil was not removed,and the body fluid was difficult to recover.Therefore,relying solely on drinking water can not alleviate,only dispelling pathogens and moistening dryness can generate fluid.Sangxing Decoction has the effect of soothing the wind and eliminating dryness,moistening the lung and generating fluid.It also indirectly further shows that there is a corresponding relationship between the formula of "measuring with formula" and the syndrome of this experimental model.