Study On The Establishment Of Yunnan Spring Dry Animal Model Based On "measured In Square Proof"

Objective:To investigate the feasibility of the construction of Yunnan Chunzao animal model based on the "measured in square proof" method by observing the changes of the biological characteristics and body fluids of mice before and after administration in the spring dry environment in Yunnan.Methods:According to the internal theory of traditional Chinese medicine,the experimental mice were randomly divided into normal control group,Chunzao group,Chunzao whit-water group,Sang Xing Tang group,Sha Shen Mai Dong Tang group and Hefang group,using artificial climate chamber.And Xiangzao food simulated the environment of Yunnan Chunzao and acted on each group of experimental mice to observe the changes of the general biological characterization of the mice in each group.The pathological morphology of the skin tissue,lung tissue,and large intestine tissue of each group was observed by HE staining.The changes were recorded.The dry and wet weights of the dorsal skin,lungs,large intestine tissues,and stools of each group were recorded to calculate the changes in water content,daily weight,diet,and water intake of each tissue.Results:(1)The mice in the normal control group had no fighting phenomenon,hair luster,and slightly wet texture;the mice in the spring-drying group and the water-dried water group had a fighting phenomenon from the fifth day after the application of the factor,and obvious scars were observed on the tail.And gradually showed irritability and irritability,the hair gradually sparse,lack of luster,the quality of the stool was slightly dry;Mulberry Decoction group mice in the application of factors after the fur shiny,occasional fighting phenomenon,later tends to calm,constipation Slightly moist;Sha Shen Mai Dong Tang group and the combination of mice during the breeding period without fighting phenomenon,hair luster,slightly moist texture.(2)Compared with the normal control group mice:1 The weight of mice in the CKD group was decreased but there was no significant difference(P > 0.05).Dietary intake per 10 g body weight decreased but there was no significant difference(P >0.05),and the water consumption per 10 g body weight was significantly different(P< 0.05)Pathological changes in lung tissue,water content in lung tissue decreased(P<0.01),water content in large intestine tissue decreased(P <0.01),water content in back skin was significantly higher than that in normal control group.Statistically,the water content in the stool decreased(P <0.01);2 The body weight of the mice in the Chunzao Shui group decreased but there was no significant difference(P > 0.05),and the diet amount per 10 g body weight decreased but there was no significant difference(P > 0.05).The water intake of 10 g body weight increased significantly(P<0.05),the histopathological change of lung tissue,the water content of lung tissue decreased(P <0.01),the water content of large intestine tissues decreased(P <0.05),and the water content of back skin decreased.(P <0.05),the water content in the stool decreased(P <0.01);3 The body weight of mice in Sangxing Decoction increased but there was no significant difference(P >0.05).There was a significant difference in the dietary intake per 10 g of body weight(P <0.05),no significant reduction in water consumption per 10 g of body weight Differences(P >0.05),slight pathological changes in lung tissue,decreased water content in lung tissue(P >0.05),decreased water content in large intestine tissue(P >0.05),decreased water content in back skin(P >0.05),stool The moisture content decreased(P >0.05);4 The weight of the mice in the Adenophora Ophiopogon Decoction group decreased but there was no significant difference(P >0.05).Every 10 g of body weight diet decreased but there was no significant difference(P >0.05),every 10 g There was no significant difference in weight drinking water(P >0.05),slight pathological changes in lung tissue,decreased water content in lung tissue(P >0.05),decreased water content in large intestine tissue(P >0.05),and decreased water content in back skin(P >0.05),the water content ofthe stool decreased(P >0.05);the weight of the mice in the combination group was decreased but there was no significant difference(P >0.05),and the diet amount per10 g of body weight decreased but there was no significant difference(P >0.05).There was no significant difference in water consumption per 10 g body weight(P >0.05),slight pathological changes in lung tissue,decreased water content in lung tissue(P >0.05),decreased water content in the large intestine tissue(P >0.05),and water content in back skin.The rate of decline(P >0.05),stool water content decreased(P >0.05).(3)Compared with the mice in the spring-dried group: 1 The body weight of the mice in the spring-dried water group decreased slightly but there was no significant difference(P >0.05).The dietary intake per 10 g body weight decreased but there was no significant difference(P >0.05).There was no significant difference in water intake per 10 g body weight(P >0.05).The water content in the lung tissue increased slightly(P >0.05),the water content in the large intestine tissue increased slightly(P >0.05),and the water content in the stool decreased slightly(P >0.05);2 Sang apricot decoction group mice body weight increased slightly but no significant difference(P >0.05),every 10 g body weight diet increased but no significant difference(P >0.05),every 10 g weight loss of drinking water no significant difference(P >0.05),the water content of lung tissue increased(P <0.01),the water content in the large intestine increased(P <0.05),and the water content in the stool increased(P<0.05);3 The weight of mice in the Radix Ophiopogon japonicus group slightly increased However,there was no significant difference(P >0.05),there was a significant difference in the dietary intake per 10 g of body weight(P <0.05).There was no significant difference in the water intake per 10 g of body weight(P >0.05),and the lung tissue water content increased(P >0.05),the water content of the large intestine increased(P <0.05),and the water content of the stool increased(P <0.05);4combination group mice slightly increased body weight but no significant difference(P >0.05),every 10 g body weight diet decreased significantly(P <0.05),every 10 g weight loss of drinking water was no significant difference(P >0.05),the water content of lung tissue increased(P <0.01),the water content of the large intestinetissue increased(P <0.05),and the water content of the stool increased(P <0.05).Conclusion:1.All the experimental animals under the spring-dried environment in Yunnan showed different degrees of dry characterization,and the dry character of the mice after the intervention of Zhishao Prescription was lighter.It is suggested that the mouse feels dryness,dryness,dryness,and dryness,so the mouse showed clinical manifestations of dryness syndrome.The moistening drug can improve the dryness of mice,and can be used to test the syndrome.It also suggests that the mouse model has the clinical features of the dryness syndrome.2.All groups of experimental animals in the spring drying environment in Yunnan had different degrees of tissue water content decline and pathological changes of lung tissue.It is suggested that the mouse feels dryness,dryness,injury to the lungs and stomach,so the mouse has a clinical manifestation of external dryness or internal and external dryness.The improvement degree of each index in the treatment group was better than that of the negative group,suggesting that the moistening drug can improve the dryness of the mice and the simple drinking water in the spring dry environment can not effectively relieve the dryness and injury of the lung and stomach.The square test showed that the mouse model has the clinical features of dryness or internal and external dryness.3.According to the principles of prescriptions,comparing the biological characteristics of mice and the changes of the lungs(skin,lung,and large intestine)before and after administration in the environment of spring drying,macroscopic signs and microstructures can be used to preliminarily infer the spring drying environment in Yunnan.The animals in the following groups basically conformed to the animal model of dryness syndrome,that is,the syndrome model of Yunnan Chunzao.