Preliminary Study On The Molecular Mechanism Of FENDRR In The Development Of Lung Cancer In Xuanwei Area

Objectives:The high incidence of lung cancer in Xuanwei area of Yunnan has the characteristics of high incidence of non-smoking women and younger diseased groups,but lacking effective diagnosis and treatment indicators is one of the most important problem.Based on the previous stage,the purpose of this study was to investigate the mechanism of action of differentially expressed IncRNA FENDRR in lung cancer in Xuanwei area.Methods:By using biochips to detect 23 cancer tissues and paracancerous tissues from lung cancer patients in Xuanwei area,screen differentially expressed IncRNA and mRNA,and further construct a differential expression gene co-expression network through bioinformatics analysis to further screen IncRNA FENDRR.Target gene.We have collected 10 pairs of cancer and adjacent tissues of lung cancer patients in Xuanwei area and extract total RNA from the tissues.The expression levels of IncRNA FENDRR and target genes in 10 pairs of tissues were detected by real-time fluorescent quantitative PCR to verify the results of the chips.Based on the ceRNA(competing endogenous RNAs)hypothesis,the Xuanwei lung cancer cell line XLA-07 overexpressing and interfering with FENDRR was constructed in vitro to further verify the correlation between FENDRR and target gene expression levels.Through the combination of cell biochip and database search,predict the mechanism of action of FENDRR to regulate target genes.Results:Through the detection and analysis of biochips,calculate the differential expression of the gene log2|Fold Change(linear)|?2 and p-value<0.05,a total of 995 differentially expressed IncRNAs were screened,and 396 of the IncRNAs with up-regulated expression were differentially expressed.There were 1948 mRNAs,of which 724 were up-regulated and 1224 were down-regulated.Combined with biochip assay results and bioinformatics analysis,the target gene KLF4 of lncRNA FENDRR was screened.The expression levels of FENDRR and KLF4 in lung cancer patients and adjacent tissues of Xuanwei area were detected by real-time quantitative PCR.The results were consistent with the results of microarray detection.FENDRR overexpression and interfering cell lines were constructed in vitro,and the expression levels of KLF4 and FENDRR were consistent by quantitative real-time PCR.Combined with cell expression profiling and database retrieval,FENDRR was screened by ceRNA pattern on hsa-miR-424-5p,hsa-miR-30a-5p,hsa-miR-200c-3p,hsa-miR-10a-5p and other genes to regulate the expression of KLF4,and through this way we can prove the mechanism of action of FENDRR in lung cancer in Xuanwei area.Conclusion:The differentially expressed IncRNA and mRNA in lung cancer tissues of lung cancer patients in Xuanwei area were detected by biochip,and the target gene KLF4 of IncRNA FENDRR was further screened.Quantitative Real-time PCR was used to verify the results of the screening of the chips.The results showed that the results were reliable.LncRAN FENDRR may regulate the expression level of KLF4 by competitively binding genes such as hsa-miR-424-5p,hsa-miR-30a-5p,hsa-miR-200c-3p,hsa-miR-10a-5p,as a result,the prognosis of lung cancer patients in Xuanwei area is poor.