| Objective:Lung cancer is one of the most rapidly growing malignant morbidity and mortality rates in the world.Xuanwei in Yunnan Province is a high-risk area of lung cancer in China,and according to related research shows:in Xuanwei,the average mortality rate of male lung cancer patients is 98.10/100000,and the average mortality rate of female lung cancer patients is 83.28/100000.Among them,non-smoking women have high mortality rate of lung cancer and can reach 120/100000.According to recent surveys,the male and female mortality rate in Xuanwei is 3-6 times that of the country's rural areas.The main reason for the high mortality rate of lung cancer among people in the Xuanwei area is the difficulty of early diagnosis.Most of the patients diagnosed are in advanced stages and lack effective treatment methods.Therefore,it is necessary to explore molecular diagnostic markers related to lung cancer in Xuanwei area as soon as possible to provide assistance for clinical early diagnosis and treatment.At present,more and more studies have found that LncRNA can regulate gene expression levels on multiple levels,and it plays an important role in the occurrence,development,and metastasis of tumors.This study examined the expression of IncRNA FENDRR in lung cancer cell XLA-07 in Xuanwei area and to investigate the effect of overexpression of FENDRR gene on the proliferation,migration,invasion and apoptosis of lung cancer cell XLA-07 in Xuan Wei area.Methods:1.Extracting the lncRNA FENDRR from lung cancer cell XLA-07 in Xuanwei population and detecting FENDRR expression by real-time quantitative PCR.2.Construction of lentiviral vectors overexpressing the FENDRR gene.After virus purification,the plasmid was packaged to produce infectious virus particles.The lentiviral plasmid was sequenced and the virus titer was detected.XLA-07 cells were infected and transfected with virus particles,and the transfection efficiency was measured by real-time fluorescence quantitative PCR.3.The cell proliferation was detected by CCK8 assay.4.The cell migration ability was detected by scratching method.5.The cell invasioness was detected by Transwell assay.6.Apoptosis was detected by Annexin V/PI double staining cells.Results:1.Real-time fluorescence quantitative PCR detection:the expression of FENDRR gene in XLA-07 cells was significantly up-regulated in the LV-FENDRR overexpressed group compared with the XLA-07 control group and the N-FENDRR no-load group(P<0.05).There was no significant difference in the changes of FENDRR gene expression in the empty and control groups(P<0.05).2.Overexpression of FENDRR results showed that the CCK8 method had a positive relationship between the number of viable cells at the wavelength of 490nm and absorbance.The absorbance of the LV-FENDRR overexpression group was lower than that of the XLA-07 control group and The N-FENDRR empty vector group,indicating LV-FENDRR.The proliferation rate of lung cancer cell XLA-07 in the Xuanwei population in the overexpression group was inhibited(P<0.05);overexpression of FENDRR reduced the hunting ability of XLA-07cells;and the invasion ability of tranwell cell invasion group decreased significantly compared with the control group and the empty body group(P<0.05).The invasion ability of the control group and the empty carrier group was not significantly different(P>0.05),and the overexpression of FENDRR promoted the early apoptosis of XLA-07 cells.Conclusion:LncRNA FENDRR can inhibit the proliferation,migration,invasion and promote apoptosis of XLA-07 cells.It has a obvious anti-cancer activity in lung cancer among the population in Xuanwei area,and it is expected to be a potential target for the prevention and treatment of lung cance... |
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