The Expression And Biological Functions Of LncRNA FENDRR In Xuanwei Lung Cancer Cells

Objectives:The studys to investigate the expression of lncRNA FENDRR in Xuanwei lung cancer cells,and to investigate the effect of FENDRR gene expression on the proliferation,migration and invasion of lung adenocarcinoma cell line XWLC-05 in Xuanwei,Yunnan.Based on microarray bioinformatics analysis by high-throughput sequencing Xuanwei lung cancer patients' tissues and adjacent normal control tissues,we explored dysexpression of lncRNA genes which caused in the process of deterioration of function changes in lung cancer,and wish to lay the foundation for the further research on lncRNA FENDRR effects to target gene during occurrence and development of Xuanwei lung cancer.Methods:1.The extraction of lncRNA FENDRR expression in XWLC-05 and A549 lung cancer cell by using qRT-PCR analysis;2.We built all gene synthesis eukaryotic expression recombinant plasmid(FENDRR gene overexpression)transfect to XWLC-05 cells.Then we built Lentivirus packaging shRNA particles,it has been puried and packaged which is a kind of ready-to-use virus.After sequencing and identification,poison to shuttle plasmid virus is transfected into XWLC-05 cells.3.By using cell proliferation MTS,Transwell diverticulum,Matrigel methods to detect cell migration and invasion functions;4.The FENDRR in Xuanwei lung cancer potential biological functions were analyzed and predicted those target genes by UCSC database and Starbase v 2.0 software that on basis of micRNAs and lncRNA function prediction.It will be meaningful to adjusting role of FENDRR in Go Terms and comprehensive evaluation.Results:1.Compared with NSCLC A549 cells,the expression of lncRNA FENDRR in XWLC-05 in cells is lower.Real-time quantitative PCR detection is that 0.74E-04±0.0lvs 13.60E-04±0.01,P<0.05;2.After post-processing transfection,quantitative PCR showed that FENDRR mRNA expression has differences between XWLC-05 which was statistically significant(P<0.05),but not as LV3-FENDRR groups(P>0.05);3.XWLC-05 was transfected pEX-3-FENDRR overexpressing plasmid.In results,we suggested that 490 mm absorbance value in 0,12,24,36 h groups was decreased that living cells have a growth inhibition.PEX-3-FENDRR XWLC-05 cell transfection group migration abilities and breaking abilities through the basement membrane of three-dimensional structure have been obviously decreased and it demonstrated that overexpression of IncRNA FENDRR can inhibit cell migration and invasion potential.4.Retrieved from the reverse transcription of FENDRR mRNA sequences overlapping in larger pieces of genetic promoter region.There are 202,including chain gene and promoter sequences,have took justice on screen.Above 90%coincidence degree,we got 7 target genes,respectively,BTD?PDGFD?RP11-359G22.4?GNG4/mir5096?SYCP1?AGBL3?ANKRD6/LYRM2.Also,transcription factor prediction analysis found that FENDRR may be associated with UPF1 dry differentiation factors.In addition,the ceRNA biology function analysis showed that FENDRR gene in GO:0010467 obviously enriched,and might interact with miRNAs related CCTN2,LSM11,MAPKAPK2,POU2F1 genes possiblly.Conclusion:LncRNA FENDRR in Xuanwei lung cancer has obvious anticancer activity which can by regulating in gene expression.It could inhibit tumor cell proliferation,migration and aggression;it may involve in the potential biological effects of lung cancer of FOXF1,CCTN2,PDGFD and other target genes possibly.