T-UCR Expression Profile Of Lung Cancer In Xuanwei,China

ObjectiveThe lung cancer in Xuanwei(LCXW),China,has distinctive characteristics and its morbidity and mortality are highest in China and are on the rise.The grim situation necessitates the exploration of its pathogenesis.T-UCRs transcribed from UCRs,which are genomic sequences with completely conservation among human,rat,and mouse genomes.Given UCRs extreme conservation,it has been speculated that these regions have important biological functions.Recent studies shown T-UCRs dysregulated frequently and the aberrant expression of T-UCRs played important roles in human cancers.Different cancer types have their unique T-UCR expression profiles.However,there is no research reporting about T-UCR expression profile in LCXW.Therefore,we aimed to explore T-UCR expression profile in different stages of LCXW and wish to lay a foundation for the further functional research of T-UCRs and provide new insights and ideas for the mechanism research of LCXWMethodsThe 26 paired LCXW and non-cancerous lung(NCL)tissues were divided into 3 groups according to their pathological stage.We carried out the T-UCR microarray to detect T-UCR expression profile and screen out differentially expressed T-UCRs and related genes in different staged LCXW.Then,RT-qPCR was used to validate the microarray data on 50 paired LCXW and NCL tissues.Finally,SPSS22.0 statistical software was used to analyze the association between the difference expression of candidate T-UCRs and patients' clinicopathological factors.ResultsT-UCRs and related genes significantly dysregulated in LCXW lung cancer tissues by comparison with the corresponding NCL tissues and the number of dysregulated T-UCRs and related genes present an increasing trend from I stage to III stage LCXW specimens.RT-qPCR analysis showed that uc.63-and uc.280+ were up-regulated.The relative expression levels of uc.234+and uc.208-,uc209-had no significant difference between the LCXW tissues and the NCL tissues.ConclusionOur study revealed the expression profile of T-UCRs and demonstrated that T-UCRs and related genes were aberrantly expressed in different staged LCXW.These T-UCRs and related genes may involved in the carcinogenesis of LCXW,which provides a basis for further revealing the mechanism of LCXW.Further study of these T-UCRs may provide new targets and methods for the diagnosis and therapies of LCXW.