Regulatory Effect Of GubenFangxiao Decoction On Expression Of TLR4 And TLR7 In Mice With Asthma Remission

Objective:Observing the regulatory effect of Guben Fangxiao decoction on Toll-like receptor(TLR)4,TLR7,nuclear factor-kappa B P65(NF-?B p65)and NF-?B inhibitor protein alpha(I?B?)in mice with asthma remission,in order to explore the mechanism of GubenFangxiao Decoction in treating asthma remission.Methods:Respiratory syncytial virus(RSV)combined with chicken ovalbumin(OVA)was used to build asthma remission model in 3-week-old BALB/c mice.Sixty mice were divided into blank group,model group,dexamethasone groups(0.001 g/kg)including low,medium and high-dose GubenFangxiao decoction group(6.5,13,26g/kg),respectively.Intervention was given once a day for 28 days.After administration,the mice were put to death.Hematoxylin-eosin(HE)staining was used to observe the pathological changes of lung tissue and score the pulmonary inflammation.Western blot was used to detect the expressions of TLR4,TLR7,I?B? and nuclear NF-?B p65 in lung tissue of mice.Immunofluorescence was used to observe the expression of NF-?B p65 in cellnucleuses.Results:Compared with blank group,the lung tissue of model group showed obvious inflammatory cell infiltration(P<0.01)and bronchial stenosis,the nuclear distribution of NF-?B p65 increased significantly(P<0.01),and the expressions of TLR4,TLR7 and I?B? proteins increased significantly(P<0.05).Compared with the model group,Guben Fangxiao decoction could significantly alleviate the inflammatory infiltration of lung tissue in mice(P<0.01),reduce the expression of nuclear protein NF-?B p65(P<0.01),relieve the nuclear entry of nuclear protein NF-?B p65(P<0.01),and increase the expressions of TLR4,TLR7 and I?B? proteins(P<0.01).Conclusion:Guben Fangxiao decoction can alleviate airway inflammation,and its therapeutic effect may be achieved by regulating TLR4,TLR7,NF-?B p65 and I?B?.