Effects Of Zhenwu Soup On The "AVP-V2R-AQP2" Pathway In Kidney-yang Deficiency Rats

Objective By observing the changes of“AVP-V2R-AQP2”pathway and the effects of Zhenwu soup on water metabolism disorder in kidney-yang deficiency to reveal the mechnasim of metabolism disorder in kidney-yang deficiency rats and the role targets of Zhenwu soup.Methods The SD rats were randomly divided into the normal contral group and the kidney-yang deficiency model groups(n=10).The kidney-yang deficiency rats were treated with adenine once per day for 21 days.The rectal temperature,holding power,weight and general symptoms and signs of kidney-yang deficiency including erect hair,back arched,curled up,grab irritation were observed per 4 days,drinking,urine and diet were detected per week.The urine of third week was collected to detect the content of 24 hour U-TP and 17-OHCS.Serum was centrifuged by Orbital blood to detect the content of T3?T4?T?E2?ACTH?CORT?BUN?Scr.Compare the all indexes to decide whether the model was successful.Then regroup the model group into the kindney-yang deficiency model group,Zhenwu so up group,Shenqiwan group.Zhenwu soup groups were treated with 22.68g/kg raw herbs of zhenwu soup per day for 21 days.The rectal temperature,holding power,weight and general symptoms and signs of kidney-yang deficiency including erect hair,back arched,curled up,grab irritation were observed per 4 days,drinking,urine and diet were detected per week and the 4 hours urine was also detected in the last week.The urine of third week were collected to detect the content of 24 hour U-TP and 17-OHCS.On the 21th,rats were anesthetized,and blood was collected by heart punctures and separated for serum to detect the contents of ACTH,CORT,AVP;hypophysis homogenates were prepared for the detection of the content of CRH;renal medullar homogenates were prepared for the contents of AQP2,V2R,cAMP,PKA All the detections in homogenates were performed by ELISA assay.The renal tissue was stained with HE and pathological changes were observed under microscope.Results 1.Compared with the normal contral group,the weight grew slower,the kidney yang deficiency model rats,rectal temperature,holding power decreased,urine increased and general symptoms and signs of kidney-yang deficiency including erect hair,back arched,curled up,grab irritation came out in the model group,and the content of T3,T4,T,E2,ACTH,CORT in the serum and 17-OHCS significantly decreased,the content of BUN,Scr,U-TP significantly increased.After HE staining,glomerular congestion,cysts expand,interstitial fibrosis and inflammatory cell infiltration,cloudy swelling of renal tubular epithelial cells,tubular obstruction and partial lumen protein exudation were observed in model group.2 After the treatment of Zhenwu soup for 21 days,the syndrome of kidney-yang deficiency improved such as weight faster,rectal temperature,holding power higher and so on The pathological examination also improved.The content of ACTH,CORT,17-OHCS,V2R,AQP2 all significantly increased and the content of Scr,U-TP all significantly decreased compared with the model groupConclusion 1.Zhenwu soup can effectively improve the function of kidney and indocrine system of kidney yang deficiency rats by improvng the content of HPA axis and 24-UTP,Scr and BUN.2.Zhenwu soup can effectively improve the general symptoms and signs of kidney-yang deficiency rats,can upregulate the content of AQP2 and correct the water metabolism disorder of kidney-yang deficiency.3.The effect of Zhenwu soup and Shenqiwan correcting the water metabolism disorder of kidney-yang deficiency is related with the multi-targets regulating "AVP-V2R-AQP2" pathway.