Expression Of MicroRNA-145,SOX2,and OCT4 In Double Primary Endometrioid Endometrial And Ovarian Carcinomas

Objective: The aim of this study was to assess the involvements of SOX2,OCT4,and microRNA-145 in the tumorigenesis of double primary endometrioid endometrial and ovarian carcinomas(DPEEOCs).We also tried to find more clonal evidences for DPEEOCs.Methods: In our study,twenty DPEEOC patients were chosen.Ten metastatic double endometrioid endometrial and ovarian carcinomas(MDEEOCs)were also chosen.Ten normal endometrial tissues and ten normal ovarian tissues were also included.All samples were paraffin tissue specimens.The expression of microRNA-145 was detected by real-time quantitive PCR.We also analyzed the correlation and differences of microRNA-145 in the double two cancer sites of DPEEOC.Differences of microRNA-145 expression in DPEEOC and MDEEOC were also analyzed.Immunohistochemical staining(IHC)was used to measure the expression of OCT4 and SOX2 in DPEEOC tissues,MDEEOC tissues,and all normal tissues.As for those positively expressed tissues,three IHC pictures were chosen and quantitatively analyzed with Image Pro Plus.We also analyzed the differences of microRNA-145,SOX2 and OCT4 in different tissues.The correlation of microRNA-145,SOX2 and OCT4 in the same tissues were also analyzed.Results: The results showed that microRNA-145 was abnormal expressed in all DPEEOC and MDEEOC tissues.It was lower expressed in DPEEOC endometrial tissues while higher expressed in DPEEOC ovarian tissues compared to corresponding normal tissues.Both SOX2 and OCT4 were positively expressed in all cancer tissues,While they were negatively expressed in normal endometrial and ovarian tissues(one NOT tissue with faint yellow staining in less than 5% ovarian cells,while it was negtively expressed according to the standard).MicroRNA-145,SOX2,and OCT4 were expressed at similar levels in two cancer sites of a given DPEEOC or MDEEOC samples(all P > 0.05).We also found a positively correlation between the expression of SOX2 and OCT4 in both two cancer sites of DPEEOC samples;the statistical results in DPEEOC samples were as follows:(endometrial cancer tissues:r=0.449,p=0.047;ovarian cancer tissues: r = 0.449,P = 0.047).However,we did not found a correlation between the expression of microRNA-145 and SOX2 or OCT4(both P >0.05).SOX2 and OCT4 were also expressed in MDEEOC lesions;the statistical were as follows: endometrial cancer tissues,r = 0.770,P = 0.009;ovarian cancer tissues :r = 0.716,P = 0.020.Besides,MDEEOC sections expressed a higher level of SOX2 and OCT4 compared to corresponding DPEEOC tissues(P<0.05).However,no difference of microRNA-145 was found betweencorrespond cancer tissues of DPEEOC and metastic DEEOC(P > 0.05).Conclusion: We thought microRNA-145,SOX2,and OCT4 might play similar roles in the tumorigenesis of the two cancer sites in DPEEOCs.Our results were in accordance with other researchers' reports about the clonality of DPEEOCs.Besides,we also found that SOX2 and OCT4 may have some implication in DPEEOC' and MDEEOC' diagnosis.