Sox2, Oct4, Twist And YB-1 Protein Expression In Cervical Squamous Cell Carcinoma Of The Research

Objective:This experiment using immunohistochemical technique(SP ldpe-g-nvp) of SSox2、Oct4 、 Twist and YB- 1 protein positive expression in cervical lesions organizations,diseased tissue according to the degree of pathological changes, divided into five groups,respectively, chronic cervical inflammation, the atypical hyperplasia lesion group(mild atypical hyperplasia namely CINI class, mild atypical hyperplasia namely CINII level,severe atypical hyperplasia namely CINIII) and cervical squamous cell carcinoma group, is to explore the four kinds of protein in cervical squamous cell carcinoma of the mechanism and significance of judgment biological behaviour, analyze their relationship with the clinical pathologic factors at the same time, provide a new reference for the prognosis of patients with cervical cancer and treatment index or targets.Methods:Collect chronic cervicitis, CINI class, CINII, CINIII and cervical squamous cell carcinoma cases, the number of cases, respectively, 20 cases, 20 cases, 18 cases, 16 cases,66 cases, using immunohistochemical SP 2 footwork, Sox2、 Oct4、Twist and YB- 1protein positive expression was studied in various cases, by the statistical analysis of these four groups of lesions in the group that protein positive expression, as well as the four proteins with cervical squamous cell carcinoma in patients with clinical pathologic factors(age, clinical stage, pathological grade, nodal metastasis).Results:1. Sox2 protein positive expression rate in chronic cervical inflammation group was10%, CIN Ⅰ group 30%, CIN Ⅱ group 50%, CIN Ⅲ group and cervical squamous cell carcinoma(87.7%, 77.7% and chronic cervical inflammation group and CIN Ⅲ group,cervical squamous cell carcinoma of the significant differences between groups, CIN Ⅰgroup and CIN Ⅲ group, cervical squamous cell carcinoma of the significant differences between groups, CIN Ⅱ groups with cervical squamous cell carcinoma of the significant differences between groups, compared with no significant differences between groups.2. Oct4 protein positive expression rate in chronic cervical inflammation group was5%, CIN Ⅰ group 40%, CIN Ⅱ group 56.2%, CIN Ⅲ group and cervical squamous cell carcinoma(75.7%, 66.7% and chronic cervical inflammation group and CIN Ⅱ group,CIN Ⅲ group, cervical squamous cell carcinoma of the significant differences between groups, CIN Ⅰ group and the cervical squamous cell carcinoma of the significant differences between groups, compared with no significant differences between groups.3. The positive expression rate of Twist protein in chronic cervical inflammation group was 10%, CIN Ⅰ group 30%, CIN Ⅱ group 43.7%, CIN Ⅲ group and cervical squamous cell carcinoma(71.2%, 61.6% and TWIST protein positive expression in chronic cervical inflammation group and CIN Ⅲ group, cervical squamous cell carcinoma of the significant differences between groups, CIN Ⅰ groups with cervical squamous cell carcinoma of the significant differences between groups, to compare the differences between groups have no statistical significance.4. YB- 1 protein positive expression rate of chronic cervical inflammation group was5.0%, CIN Ⅰ group 25.0%, CIN Ⅱ group 50.0%, CIN Ⅲ group and cervical squamous cell carcinoma(81.8%, 55.5% and YB- 1 protein expression in chronic cervical inflammation group and CIN Ⅲ group, cervical squamous cell carcinoma of the significant differences between groups, CIN Ⅰ exist significant differences between group and cervical squamous carcinoma group, there was no statistically significant difference compared between groups.5. Sox2 protein positive expression and cervical squamous cell carcinoma of the different age groups and the presence of lymph node metastasis in patients with nostatistical significance, Sox2 protein positive expression and the patient’s different clinical stage and pathologic differentiation degree have statistical significance; Oct4 protein positive expression and cervical squamous cell carcinoma patients with different age groups has no statistical significance, Oct4 protein positive expression and the patients with different clinical stage, pathological differentiation degree of different, and presence of lymph node metastasis was statistically significant; Twist protein positive expression and cervical squamous cell carcinoma in patients with different age groups and different clinical stages and pathological differentiation degree of no statistical significance, Twist protein expression and the presence of lymph node metastasis was statistically significant;YB- 1 protein positive expression and cervical squamous cell carcinoma patients of different age groups and different clinical stages of statistical significance, YB- 1 protein positive expression and histopathological differentiation degree, and presence of lymph node metastasis was statistically significant. Statistical significance was found(P < 0.05,P > 0. 05 without statistical significance)Conclusion:1.In chronic cervical inflammation, the atypical hyperplasia lesion group(CINI class,CINII, CINIII) and Sox2 in cervical squamous cell carcinoma group, Oct4, Twist and YB-1 protein positive expression rate of all showed a trend of increasing gradually, prompt the study of the four proteins closely associated with cervical squamous cell carcinoma cancer mechanism.2.Sox2,Oct4,Twist and YB- 1 protein positive expression rate and cervical squamous cell carcinoma is closely related to the clinical pathologic factors, studying the protein expression can be used to monitor the malignant degree of cervical squamous cell carcinoma and is important for the treatment of patients with invasion, metastasis and the role of.