Functions Of Heat Shock Transcription Factor AtHSFA6b Of Arabidopsis In Regulated Stress Resistance

Arabidopsis thaliana,as a model plant,most of the gene functions have been extensively studied.However the function of heat-shocked transcription factors in biotic and abiotic stress is not very clear.In this study,AtHSFA6 b overexpression and RNAi transgenic plants were identified,and the function of AtHSFA6 b where to study different stresses.Arabidopsis thaliana was treated in high temperature,low temperature and drought.Real-time PCR results showed that AtHSFA6 b gene was induced by abiotic stresses.AtHSFA6 b was induced with flag22 and chitin treatment.AtHSFA6 b overexpression plants were more tolerant to high temperature,low temperature and drought,while AtHSFA6b-RNAi plants were significantly less tolerant to these stresses.Compared with Col,the resistance of the AtHSFA6 b overexpression plants were enhanced to Pseudomonas syringae pv.tomato DC3000,Pseudomonas syringae pv.maculicola ES4326 and Botrytis cinerea,while the AtHSFA6b-RNAi plants were more susceptible to the pathogens.The transcription level of disease-resistance-related genes in plants was detected by PCR,The transcription level of disease-resistance-related genes in AtHSFA6 b overexpression plants was significantly up-regulated,whereas the transcription level of disease-resistance-related genes in AtHSFA6b-RNAi plants was significantly lower,indicating that AtHSFA6 b gene is involved in disease resistance and plays an important role in resistance to stress.Our results indicate that AtHSFA6 b gene is induced by abiotic and biotic stress,and plays an important role in plants' resistance to different stresses.At the same time,AtHSFA6 b gene is induced by flage22 and chitin,which plays a certain role in plant PTI reaction.