Influence Of Arabidopsis NPR1Over-expression On Disease Resistance In Brassica Napus L.

Brasicca napus L is one of the three major oil crops in the world, and is an important source of vegetable oil and vegetable protein. However, during the long process of cultivation, lots of diseases have been found to reduce seriously the production and the quality of rapeseed and lead to a huge economic losses.Nonexpressor of pathogenesisrelated gene1(NPR1) is one of the crucial genes related to disease resistance in plants. It does not only regulate basic resistance and resistance decided by resistance genes(R), but also leads to systemic acquired resistance (SAR) and induced systemic resistance (ISR). Signal molecules such as Salicylic Acid(SA), jasmonic acid(JA) and ethylene that induce acquired resistance in plants can be regulated by NPRl. The expression of Pathogenesis-Related (PR) genes can be enhanced by NPRl to improve the disease resistance in plants. It is very promising touse genetic engineering for plant protection.This study is a collaboration with Duke University, USA. In this study the Arabidospsi NPRl overexpression construct PBI1.4t from Duke University was introduced into Brassica napus by using the method of Agrobacterium-mediated transformation. Molecular staudy and disease-infection study indicated that we have got transgenic rapeseed with improved disease resistance. NPT Ⅱ gene and NPRl gene of the PCR by transformed plants, the preliminary view that has been genetically modified Brassica napus plants. Evaluation of disease resistance of the TO generation Brassica napus by the result showed that TO transgenic plants showed high resistance.