Analysis Of Stress Tolerance Of SlNAC7 Transgenic Arabidopsis And Its Mechanisms

NAC(NAM,ATAF1/2,CUC2)transcription factors play an important role in plant growth and development and in response to abiotic stress.A NAC transcriptional factor(SlNAC7)gene(NAC)was cloned in the early laboratory of the salted plant.Two Sl NAC7 transgenic Arabidopsis with the previous obtained(L1,L2),wild type Arabidopsis(WT)and empty vector(pBI121-GUS)of Arabidopsis thaliana as material,the abiotic stress treatment,analysis of SlNAC7 transgenic Arabidopsis resistance and resistance mechanism analysis.The main results are as follows:1.Under normal culture conditions,fresh weight of transgenic Arabidopsis thaliana,wild Arabidopsis thaliana and transferred Arabidopsis was measured.It was found that the average fresh weight of transgenic SlNAC7 Arabidopsis was higher than that of Arabidopsis thaliana and Arabidopsis thaliana in Sl NAC7.2.Under abiotic stress conditions,the SlNAC7 transgenic Arabidopsis,wild type Arabidopsis and transgenic vector of Arabidopsis seed germination rate,root length and survival rate,stomatal closure,chlorophyll fluorescence,relative conductivity,proline content,MDA content and antioxidant enzyme activity indicators detection and resistance analysis.Seed germination rate showed that the seed germination rate of transgenic Sl NAC7 Arabidopsis was significantly better than that of Arabidopsis thaliana and wild Arabidopsis thaliana under abiotic stress.The results of root length,survival rate and stomatal opening and closing showed that under abiotic stress,the growth status of transgenic Sl NAC7 plants was significantly better than that of Arabidopsis thaliana and Arabidopsis thaliana.The results of electrical conductivity showed that the destruction of the cell membrane of Arabidopsis thaliana under abiotic stress was less than that of Arabidopsis thaliana and the Arabidopsis thaliana in the Sl NAC7 gene.The results of chlorophyll fluorescence showed that the conversion of SlNAC7 gene to Arabidopsis under abiotic stress could improve the photosynthetic capacity of the Arabidopsis.The results of proline content,malondialdehyde content and antioxidant enzyme activity showed that transgenic Arabidopsis thaliana could enhance the tolerance to oxidative stress and cell membrane stability under abiotic stress,thereby enhancing the tolerance of transgenic Arabidopsis to stress environment.3.According to the results of stress tolerance analysis of transgenic Arabidopsis thaliana,we selected stress related genes and developmental related genes WRKY22,WRKY59,MYB96,MYB105,BHLH100,RD29 A and RD29 B to carry out real-time fluorescence quantitative PCR analysis.The results showed that these genes were up-regulated in SlNAC7 transgenic Arabidopsis thaliana,indicating that these genes may be regulated by SlNAC7 and related to the improvement of stress tolerance in transgenic Arabidopsis thaliana.