Functional Study Of Arabidopsis Thaliana Nucleoplasmic Transporter TRN1 Involved In Stress Adaptation

In Arabidopsis thaliana,TRANSPORTIN1(AtTRN1)gene can be encoded into nuclear transporter,which functions like homologous proteins in yeast cells and human cells,and participates in material transport between cytoplasm and nucleus,thus completing various life activities.At present,there are few studies on TRN1 protein,and the specific transport mechanism remains to be further elucidated.In this study,we studied the phenotypic and physiological changes of pure and weak mutant trn1-3 and sic1 under abiotic stress,such as NaCl,LiCl,KCl,Mannitol and PEG,compared with those of wild type,including inhibition of root growth,fresh weight change,expression level of antioxidant enzymes genes and determination of various enzymatic activity.At the same time,we expanded the correlation by yeast two-hybrid assay with proteins interacting with carboxyl terminal of TRN1 for further understanding.In this study,we found that under abiotic stress conditions such as high salinity of 150 mMNaCl and drought treatment,there were significant differences between TRN1-related mutants trn1-3 and sic1-weak mutants in response to stress conditions and in the expression of antioxidant enzymes genes and PR family of drought-related genes between TRN1-related mutants and wild-type ones.Through the data statistics and analysis of related physiology and molecular biology,it was found that TRN1-related mutants had certain physiological characteristics of drought resistance.Drought experiment,rehydration experiment and quantitative analysis of PR family of drought gene were carried out to further verify the results.We further verified the direct interaction between drought-related protein PR family and carboxyl terminal of TRN1 by yeast two-hybrid system,act out on SD/-Trp/-Leu/-His(TDO)+X-?-gal and SD/-Trp/-Leu/-His/-Ade(QDO)+ X-?-gal growing well.Phenotypic results and molecular experimental data showed that trn1-3 mutant showed a more significant drought-tolerant phenotype.Further studies on the physiological mechanism of drought-tolerance will be carried out in order to expand our understanding of its function.