| Objective:Collect ostiomeatal complex secretion,to observe the similarities and differences between"stagnated heat of gallbladder "type nasosinusitis patients and normal human about the nasal sinus advantages of aerobic bacteria.In the laboratory environment, to observe the growth state, growth quantity and biofilm formation ability of Staphylococcus epidermidis which isolated from clinical isolate.Artificial cultivation Staphylococcus epidermidis biofilm,to observe the inhibitory effect of Biyuanshu Oral Liquid on Staphylococcus epidermidis biofilm,the morphological changes of biofilm were observed by laser scanning confocal microscope.Beginning from the formation of biofilm of conditional pathogenic bacteria of nose and nasal sinuses,to further research the effect mechanism of "clearing gallbladder and relieving heat method"on the treatment of CRS.Discuss the clinical significance of the "The Nasal Five-du Syndrome Differentiation"on CRS,in order to provide more experimental basis and research ideas to it。Methods:1.Collect the secretions of ostiomeatal complex in"stagnated heat of gallbladder"type nasosinusitis patients and normal human, for conventional aerobic cultivation, isolation and identification of predominant bacteria.2.Qualitative and quantitative the changes of staphylococcus epidermidis biofilm producing ability and growth, screened the PIA positive expression strains.3.To gauge the minimum inhibitory concentration of Biyuanshu Oral Liquid against Staphylococcus epidermidis and the inhibitory effect of different concentration of Biyuanshu Oral Liquid to different periods biofilms.4.Artificial cultivation Staphylococcus epidermidis biofilm,to observe the inhibitory effect of Biyuanshu Oral Liquid on Staphylococcus epidermidis biofilm,the morphological changes of biofilm were observed by laser scanning confocal microscope.Result:1.9 of the 21 cases, the dominant bacteria were Staphylococcus epidermidis, approximately 42.9% of the total number of aerobic bacteria. There were 6 strains with the ability to produce membrane, including 5 strains of sinusitis group and 1 normal controls. approximately 66.7% of the total number of Staphylococcus epidermidis.2.The adhesive bacteria of PIA positive group were appeared in 4h, and the adhesive bacteria of PIA negative group were appeared after 8h.There was a significant difference between PIA positive group and PIA negative group (P<0.05)3.The minimum inhibitory concentration of Biyuanshu Oral Liquid for Staphylococcus epidermidis was 64 times diluted. There was no significant difference in the inhibitory effect of the concentration of 2 and 64 times diluted in different time periods(P<0.01).4.Under the LSCM:Id, a large number of bacteria adhesion, some have gathered trend.3d. the membrane was formed and the channel pattern changed, and the membrane structure was relatively loose.7d,the formation of three-dimensional membrane structure neat, bacteria group was wrapped in the dense neat membrane structure.Biofilms,which be inhibitied by Biyuanshu Oral Liquid,three-dimensional membrane structure were destroyed, and gradually disintegrated and differentiation with time increases.Conclusion:1.Staphylococcus epidermidis is the largest kind of aerobic bacteria at ostiomeatal complex.In sinusitis group,they have higher ability to produce biofilm.Qualitative and quantitative experiments showed no significant difference in the ability to judge the biofilm production.2.Adhesionis the beginning process of biofilm formation of Staphylococcus epidermidis, and 4h-8h is the key stage of biofilm formation.It can be considered that the biofilm of Staphylococcus epidermidis enters the mature stage and the maintenance stage. A large number of free bacteria are wrapped to form a stable membrane structure.3.In vitro,64 times dilution of Biyuanshu Oral Liquid can inhibit the adhesion of Staphylococcus epidermidis, with the increase of concentration, inhibition strengthened gradually, when up to 4 times diluted concentration. The inhibitory effect had no obvious change.4.Biyuanshu Oral Liquid on the nose and nasal sinuses Staphylococcus epidermidis biofilm in vitro inhibition might influence bacterial adhesion, and on bacterial polysaccharide adhesion factor decomposition. |
