The Study Of Correlation Between Effect Of Allergic Factors And Bacterial Biofilm In Patients With Chronic Rhino-sinusitis

Objective:To observe bacterial biofilm formation and morphologicalcharacteristics of nasal surface between allergic nose-sinusitis patients andnon-allergic nose-sinusitis patients by the scanning electronmicroscopy.Preliminary explore the impact of allergic factors to the expressionof bacterial biofilm in chronic nose-sinusitis patients,and observe goblet celldensity on chronic nasal-sinusitis nasal surface,Investigate the relationshipbetween goblet cells and the growth of bacterial biofilms to provide atheoretical basis for clinical prevention and treatment of chronic nasal.Method: The objects of study were24patients with chronic nasal-sinusitis who have endoscopic sinus open surgery at the Third Hospital ofHebei Medical University ENT for the period from June2012toSeptember,record the patient’s age,sex,symptoms and signs,all patientsunderwent the testing of allergy,examination of sinus CT,and the score ofLund-Mackay sinus CT and visual analog scale (VAS).Based on whether thepatients exist allergic factors will be divided into:allergic nose-sinusitis groupand non-allergic nose-sinusitis group.All patients were specimens the mucosaof uncinate process,the ethmoid bulla or maxillary sinus as specimens inendoscopic sinus surgery,specimens were observed in the scanning electronmicroscope after treatment by conventional sample preparation method ofscanning electron microscopy.Judge whether the presence of bacterial biofilmsby comparing the hundreds of pictures of bacterial biofilms in theliterature.Descripe the formation and morphological characteristics ofbacterial biofilms and analyze the difference between the allergicnose-sinusitis group and non-allergic nose-sinusitis group.Specimens haveparaffin sections, alcian blue-periodic acid-Schiff (AB-PAS)staining,and countthe number of goblet cells to analyze the differences between the allergic nose-sinusitis group and non-allergic nose-sinusitis group.Do the correlationanalysis of the presence or absence of allergic factors and part of the clinicalindicators.Each group of experimental data used SPSS13.0software packagefor statistical analysis,measurement data were expressed as mean±standarddeviation,compared of using t-test.The differences of bacterial biofilmsbetween the two groups were Compared of using Fisher exact2tables,p <0.05for the difference was statistical significant.Results:1In the24patients with chronic nasal-sinusitis,10cases (41.67%) ofpatients were allergic nasal-sinusitis,the patients with non-allergicnasal-sinusitis were14cases(58.33%).2The positive results of bacterial biofilms which were observed byscanning electron microscopy.24cases nasal sample of CRS patients whichwere observed by the scanning electron microscopy was founded that18casespatients Contain different forms of BBF, the positive rate was75.0%, of which10cases patients were found BBF in10patients with allergic nose-sinusitis,the positive rate was100%,and8cases patients were found BBF in14patientswith non-allergic nose-sinusitis,positive rate was57.1%,after statisticalanalysis,p <0.05,we think that the positive rate of bacterial biofilm of the twogroups of patients is different.3The injury of nasal mucosa and morphology of bacterial biofilm inscanning electron microscopy.All samples were observed that the cilia on themucosa surface was disorder,lodging in different degree,or even the absence,the cilia was damaged to a lesser degree in patients with negative expressionof bacterial biofilm.There are a large number of mucinous matrix on mucosalsurface in patients with positive expression of bacterial biofilm.The typicalbacterial biofilms which have water passages and a plurality of micro-colonieswere combined together to form the characteristic three-dimensional structurewas found on the mucosal surface in18patients with chronic nasal-sinusitis.These samples contain the different stages of the life cycle of bacterialbiofilms. 4the VAS scores of clinical symptoms and Lund-Mackay scores of sinusCT between the two groups were compared.The VAS scores of clinicalsymptoms in allergic nose-sinusitis group and non-allergic nose-sinusitis were21.40±8.70and20.79±6.27,compared of using t-test,p>0.05,VAS scores of thetwo groups of patients can be considered are not different.The Lund-Mackayscores of sinus CT in allergic nose-sinusitis group and non-allergicnose-sinusitis group were12.60±5.12and10.07±3.95,compared of using t-test,p>0.05,Lund-Mackay scores of the two groups of patients can be consideredare not different.5Compared with the numbers of goblet cells in the mucosal ciliabetween the two groups.The numbers of goblet cells with allergicnose-sinusitis group and non-allergic nose-sinusitis group were27.40±2.32and16.43±1.93,compared of using t-test,p<0.01,we can consider that thenumbers of goblet cells in the mucosal cilia of the two groups of patients aredifferent.Conclusion:The positive rate of bacterial biofilm in allergicnose-sinusitis group was higher than the non-allergic nose-sinusitis group,astatistically significant difference between the inferred allergic factor,It waspresumed that the existence of allergic factors was closely related to theformation of bacteria biofilm,allergic factors might be the one of the riskfactors of the formation of chronic nose-sinusitis bacterial biofilm.Thenumber of goblet cells in the allergic nose-sinusitis group was higher than thenon-allergic nose-sinusitis group,the difference between the two groups wassignificant statistically.Due to goblet cells is the main secreting cells of themucosal epithelium,and we found that bacterial biofilm-positive patients withmucosal surface had more mucinous matrix by scanning electron microscope,we can speculate that the mucinous matrix secreted by goblet cells mayprovide the nutrients needed for growth for the bacterial biofilm.Therefore,there was a positive expression rate of bacterial biofilms in patients withallergic factors. In addition,in the chronic nose-sinusitis patients,the presenceor absence of allergic factors was unrelated to VAS scores and sinus CT score.