An Study On The Relationship Between Interleukin-17 And Familial Aggregation Of Hepatocellular Carcinoma

Objective: To investigate the correlation between serum level of IL-17 and familial clustering of hepatocelluar carcinoma(HCC)by detecting the expression of cytokine interleukin(IL)-17 in the serum level between HCC high-incidence families and carcinoma-free families in Guangxi.Methods: 112 paired subjects of HCC high-incidence families(the experimental group)and those from carcinoma-free families(the control group),a total of 224 cases,in areas of high incidence of liver cancer in Guangxi were selected.There were 65 males and 47 females in the HCC high-incidence families with an average age of(27.1 ± 21.2)years and 65 males and 47 females in the carcinoma-free families with an average age of(26.8 ± 17.7)years.Among the 112 cases of HCC high-incidence family members,they were divided into first(62 cases),second(11 cases),third(25 cases),fourth and above(14 cases)class relatives according to different genetic coefficient.According to different numbers of HCC patients in HCC high-incidence families,it was divided into 2~3 cases group(70 cases)and ?4 cases group(42 cases).The levels of IL-17 in peripheral blood were detected by Enzyme-Linked Immunosorbent Assay(ELISA).Results:(1)The overall levels of IL-17 in HCC high-incidence families and carcinoma-free families were(71.13 ± 30.22)pg/ml and(19.01 ± 14.46)pg/ml,there were significant differences between the two groups(t = 16.464,P= 0.000).We further analyzed the levels of IL-17 in different characteristics(including male,female,?25 years,>25 years,HBsAg positive,HBs Ag negative,zhuang zu and yao zu)in the HCC high-incidence families members compared to those from carcinoma-free families,and it indicated that the average concentration of IL-17 was(71.16±28.43)pg/ml?(71.08±32.86)pg/ml?(69.32±29.53)pg/ml ?(73.73±31.33)pg/ml ?(70.14±20.11)pg/ml ?(71.82±35.76)pg/ml?(72.38±33.65)pg/ml?(67.99±19.25)pg/ml and(17.36±14.45)pg/ml ?(21.30±14.30)pg/ml ?(17.19±13.33)pg/ml ?(21.63±15.71)pg/ml ?(18.48±13.99)pg/ml ?(19.39±14.87)pg/ml ?(19.25±15.09)pg/ml?(18.41±12.93)pg/ml,respectively.Compared with that in the experimental group and the control group,t values were 13.603,9.524,13.072,10.081,14.307,10.999,12.886,12.094,respectively.All P values <0.05.There were significant differences between the two groups in the level of IL-17,independent from sex,age,HBsAg infection and nation.(2)There was no significant difference in the level of IL-17 among family members with different genetic coefficient in the HCC high-incidence families group(Z=4.831,P=0.185).(3)The level of IL-17 declined with the increased number of HCC patients in HCC high-incidence families,but these differences are not statistically significant(t=0.580,P = 0.563).Conclusion: There is a correlation between the increased expression of IL-17 in HCC high-incidence families and familial clustering of HCC.