The Effects Of Contact Dermatitis Induced By Acupoint Sticking Therapy On Asthma Relapse And The Expression Of T-bet/GATA-3 MRNA In Blood

Purpose:The purpose of this study is to observe the recurrence and the quality of life in bronchial asthmatic patients who have contact dermatitis induced by the treatment of Acupoint sticking therapy.And investigate the correlation between the presence of contact dermatitis and the content of T-bet mRNA and GATA-3 mRNA,by comparing the values of T-bet mRNA and GATA-3 mRNA in peripheral blood of bronchial asthma patients who has contact dermatitis or those who don’t have contact dermatitis.Methods:According to inclusion-exclusion criteria,bronchial asthma patients treated with acupoint sticking therapy during the period from June 2016 to August 2016 were observed for the study.All these patients received treatments in department of acupuncture and rehabilitation,Nanjing University of Traditional Chinese Medicine.Patients were divided into two groups,according to whether contact dermatitis appeared after acupoint sticking therapy.This study explored the relationship between contact dermatitis and asthma recurrence,by assessing the ACT scores and AQLQ scores before and after treatment.In the end,This study investigated the correlation between the presence of contact dermatitis and the content of T-bet mRNA and GATA-3 mRNA,by comparing the values of T-bet mRNA and GATA-3 mRNA in peripheral blood of bronchial asthma patients who has contact dermatitis,with those who don’t have contact dermatitis,with the application of Realtime RT-PCR method.All these people have received acupoint sticking therapy for the treatment of bronchitis.Results:Results:(1)ACT and AQLQ scores:①In contact dermatitis group,both November 2016 and February 2017 were significantly higher than those before treatment(November 2015 and February 2016)(P<0.01).The number of non contact dermatitis group increased in November 2016 compared with the same period of last year(P<0.05).②AQLQ total score:the total score of AQLQ in patients with asthma had contact dermatitis group and no contact dermatitis group.In November 2016 and February 2017,it was significantly higher than that in the same group before treatment(P<0.01).The units of AQLQ score:the limitation of activity unit in AQLQ:contact dermatitis group and non contact dermatitis group in November 2016 than the same group of the same period last year increased significantly(P<0.01)The number of contact dermatitis group and non contact dermatitis group increased in February 2017 compared with the same period of last year(P<0.05).Comparison of asthma symptom scores:contact dermatitis group in November 2016 and February 2017 over the last year and no contact dermatitis group in November 2016 compared with the same period last year which were significantly increased(P<0.01)The number of non contact dermatitis group increased in February 2017 compared with the same period of last year(P<0.05).Comparison of psychological scores:contact dermatitis group in November 2016 and February 2017 with the same period last year and no contact dermatitis group was significantly higher than the same period last year in February 2017(P<0.01)The number of contact dermatitis group in November 2016 was higher than that in the same period of last year(P<0.05).Compared to the original stimulus response score:contact dermatitis group in November 2016 and February 2017 compared to the same period last year increased significantly(P<0.01)Worry about the health of the disease:contact dermatitis group and no contact dermatitis group were increased in November 2016 and February 2017 compared with the same period before the treatment(P<0.05).(2)T-bet.GATA-3 mRNA Realtime-PCR results:①The 2-ΔCt values of T-bet mRNA in blood of patients both with and without contact dermatitis statistically decreased after acupoint sticking therapy(P<0.01).②Patients with contact dermatitis got an statistically higher T-bet/GATA-3(2-ΔCt)ratio after treatment(P<0.05).③Patients with contact dermatitis got a higher in 2-ΔΔCt value of T-bet,a lower 2-ΔΔCt value of GATA-3,but without obvious difference(P>0.05),and we find a higher T-bet/GATA-3(2-ΔCt)ratio than those without contact dermatitis(P<0.01).Conclusions:The bronchial asthmatic patients who received acupoint sticking therapy have a lower recurrence rate and higher life quality than they before the treatment.And patients with contact dermatitis get better asthma control and has better long-term effect than patients without contact dermatitis.The expression of T-bet mRNA in patients’ blood significantly increased after accepted acupoint sticking therapy.Patients with contact dermatitis had a higher T-bet/GATA-3 ratio than those without contact dermatitis.The balance is more inclined to bias to T-bet.Namely,patients with contact dermatitis have a better curative effect after treatment.