| 1 Objective::Through the experimental study on lung tissue of asthma rat model,explore Pingchuanning in regulating PI3K-Akt(intracellular phosphoinositide kinase)signal transduction mechanism,airway morphologic changes and PIP2,lung tissue of rats with cold asthma PDK1,intervention of Caspase9 related proteins in the airway remodeling in asthma.To provide the experimental basis for the application of prescription of Pingchuanning in clinic.2 Methods: Were randomly divided into two groups of 140 male SD rats(normal group,model group,guilongkechuanning group,dexamethasone group,high dose group,Pingchuanning Pingchuanning Pingchuanning middle dose group,low dose group),seven groups in each group divided into 20.Induced by 10% egg suspension and inhalation of 1% egg protein suspension,and then stimulated by cold,SD rats were subjected to cold asthma model,and finally the model was successful.At the beginning of twenty-first days,the rats were divided into different groups.According to the people and the surface area of rats on conversion,dexamethasone group,guilongkechuanning group,Pingchuanning Pingchuanning large dose group,middle dose group,low dose group of Pingchuanning equivalent dose.Seven groups were given 2ml/d,in which the normal group and model group were treated with normal saline,and the other groups were treated with the corresponding drugs.After twenty-eighth days of continuous intragastric administration,the last egg protein sensitization.After 2 hours of sensitization,the SD rats were dissected and the lung tissue was removed.The left lung and right lung were treated with different methods according to the experimental requirements.HE was used to observe the pathological changes of rat lung tissue staining,reverse transcription polymerase chain reaction(Sq RT-PCR)was detected by semi quantitative Caspase9,PIP2,PDK1 expression abundance,using immunohistochemistry to detect the expression of GH and b FGF in airway remodeling index.3 results:3.1 Lung tissue HE staining: In the model group,the airway remodeling of lung tissue was obvious,and there were a lot of inflammatory cells infiltrated around the trachea;in addition to the normal group,other groups of airway remodeling phenomenon compared with model group,there were different degree of inhibition.3.2 The expression of GH and b FGF detection of lung tissue: compared with the normal group,GH and b FGF in lung tissue of model group was significantly P<0.01;compared with the model group,the treatment group of lung tissue GH andb b FGF expression decreased,P<0.05;dexamethasone group and guilongkechuanning group,P< 0.05,there are differences;comparison of Pingchuanning large,medium and small dose,high dose and middle dose group than in low dose group,P<0.05;Pingchuanning high levels decreased significantly in GH and b FGF expression in lung tissue of superior dose group,dexamethasone group and guilongkechuanning group,P<0.05,P<0.01,have statistical significance.3.3 Semi quantitative detection of the expression of Caspase9,PIP2 and PDK1 in lung tissue: compared with the normal group,model group,Caspase9,PIP2,PDK1 in lung tissue were significantly different,P<0.01;compared with the model group,the treatment group Caspase9,PIP2,lung tissue PDK1 can down regulate the expression of P<0.05 value;comparison of Pingchuanning small dose group,high dose group and middle dose group than low dose group,P<0.05;Pingchuanning in high dose group and dexamethasone group and guilongkechuanning group,Caspase9,PIP2,PDK1 expression was significantly lower than that of dexamethasone group and guilongkechuanning group P<0.01.4 Conclusion: Pingchuanning by adjusting the cold asthma lung of rat PI3K-Akt/PKB signal transduction pathway,decreased the expression of Caspase9,PIP2,PDK1,GH,b FGF expression decreased,reduce airway hyperresponsiveness,inhibition of invasion of cold asthma rat inflammatory cells,reducing inflammatory reaction,thus delaying the airway remodeling the treatment of asthma.. |
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