Effect Of Pingchuanning On The Expression Of PKC、FKHR、Bad In PI3K-Akt Signaling Pathway In The Treatment Of Asthma

Objective:To investigate the mechanism of action of PI3K-Akt signaling pathway-related protein in the treatment of cold asthmatic rats by Pingchuanning.To detect the changes of expression levels of P21CIP1,P27KIP1,Bad,FKHR,and PKC in PI3K-Akt signaling pathway after intervention with Pingchuanning By immunohistochemistry and RT-qPCR Methods.To study the effect and mechanism of Pingchuanning on cold asthmatic rats.Method:105 healthy SD male rats(6-8 weeks,180-230 g weight)were randomly divided into 7 groups,each group of an average of 15 rats.The seven groups were normal group,model group,Pingchuanning high-dose group,Pingchuanning middle-dose group,Pingchuanning low-dose group,Guilong Kechuanning group and dexamethasone group.After adaptive feeding,except for the normal group,the other groups were sensitized on the first day and the eighth day,and the OVA and physiological saline were arranged into a 10% OVA suspension,and the rats were intraperitoneally injected,each of which was injected 1 ml.Rats were injected with 1 ml of normal saline in the normal group.On the 15 th day,each group outside the normal group was aerosolized with 1% OVA solution,and cold stimulation was given once a day,30 minutes each time,for one week in a row.On the 21 st day,the rats were intragastrically administered,and administered according to human and rat body surface area,and the model group and the normal group were given physiological saline of the same volume,continuous four weeks.After the end of the stomach,another 1% OVA ultrasonic atomization and cold stimulation were performed.Within 2 hours,the rats were anesthetized and dissected,lung tissue was taken,the left lung was placed in formalin,and the right lung was placed in Cryotube and the tube was placed in a-80℃ refrigerator for storage.The pathological changes of lung tissue were detected by HE staining.The expression levels of Bad,FKHR and PKC in lung tissue were detected by RT-qPCR.The expression levels of P21CIP1 and P27KIP1 in lung tissue were detected by immunohistochemistry.Results:The results of pathological examination showed that compared with the normal group,the bronchial structure of the lung tissue of the model group was significantly disordered,the airway epithelial cells were detached,the wall was thickened,the mucosa was congested,and inflammatory cell infiltration was observed,each treatment group has different degrees of pathological changes;compared with the model group,the pathological changes of lung tissue in each treatment group were improved.The results of immunohistochemistry showed that compared with the normal group,the expression levels of P21CIP1 and P27KIP1 in the lung tissues of other groups were significantly different(P<0.01).Compared with the model group,there were significant statistical differences in the expression of P21CIP1 and P27KIP1 of each treatment group(P<0.01).Among them,the effect of Pingchuanning high-dose group on P21CIP1 and P27KIP1 was better than other groups.The results of RT-qPCR showed that compared with the normal group,the expression levels of PKC mRNA,FKHR mRNA and Bad mRNA in the lung tissues of other groups were significantly different(P<0.01).Compared with the model group,the expressions of PKC mRNA,FKHR mRNA and Bad mRNA in rat lung tissues of each drug group were significantly different(P<0.01).The high-dose group of Pingchuanning had better effect on PKC and FKHR than other treatment groups(P<0.01).The high-dose group of Pingchuanning and dexamethasone had better effect on Bad than other treatment groups(P<0.01).Conclusion:Pingchuanning inhibits airway smooth muscle proliferation and reduces inflammatory cell infiltration in lung tissue of rats with cold asthma by regulating P21CIP1,P27KIP1 protein and PKC,FKHR,and Bad expression levels in PI3K-Akt,thereby reducing airway Remodeling,reducing inflammation to treating asthma.