The Study On Effects Of Regulating Of The Erk Signal Pathway Using Pingchuanning To TGF-β₁、Cyclind1and EGFmRNA、PDGFmRNA Expressions In The Lung Tissues Of Rats Taken Asthma Of Cold Type

1Objective By investigating the effects of Pingchuanning how to adjust airwayremodeling， ERK and Ning airway and the semi-quantitative level of lung tissue onTGF-β1, CyclinD1expression and EGFmRNA, PDGFmRNA in cold asthma model rats.Asthma intervention rather clear airway remodeling and regulation of lung tissue ERK(extracellular signal-regulated kinase) signaling pathway molecular mechanisms, ratherdialectical argumentation asthma asthma interventions and the importance of delaying,in order to better guide clinical practice.2Method2.1Experimental research105SD male rats were randomized into NormalgroupA、model groupB、the Guilong kechuanning groupC、Dexamethasone groupD、Pingchuanning high-dose group、 Pingchuanning dose group and Pingchuanninglow-dose group (E、F、G)with15in each.15rats by intraperitoneal injection of1mlsaline solution, to make the remaining90rats by intraperitoneal injection of mould first,and on the eighth day of100g/L egg albumin saline suspension liquid1ml sensitization,with cold asthma model was established. Since15days injection, the rats in the fog incylinder (normal control group with normal saline instead of) with1%egg albuminsolution ultrasonic atomizer,20-30minutes each time, once a day; at the same time putrats in adjustable cold box (0-2℃),1time/day,2hours each time,7days in a row, withSD rats appeared to nod, shortness of breath, abdominal muscle twitching, rhythmiccontraction, palpitations phenomenon such as oral cyanosis, representative model copysuccess. Building in21days, continue to spray and cold stimulation4weeks. Thenormal group, model control group given amount of saline irrigation uniform every day,GuiLong to cough and gasp the ning group, dexamethasone group, and asthma ratherhigh, medium and low dose group according to the human body and the surface area ofrats conversion than equivalent dose lavage. Lavage after4weeks anatomy of rat lungtissue, with3%sodium pentobarbital intraperitoneal injection of1ml/100g for anesthesia in rats, respectively, according to the requirements of testing materials andcorresponding treatment. HE dyeing lines are used to observe each group thepathological morphological changes of lung tissue, lung tissue was evaluated byimmunohistochemical method in expression of TGF-β1、CyclinD1, using reversetranscription-polymerase chain reaction (rt-pcr) semi-quantitative detection betweengroups EGFmRNA, PDGFmRNA, phosphorylation ERK1/2mRNA expression ofabundance.3The results3.1Experimental part3.1.1The comparison of Pingchuanning of TGF-β1、CyclinD1: There is differencebetween the model group and the normal group，P<0.01; Compared with the modelgroup, the treatment group rather asthma lung tissue TGF-β1and CyclinD1aredifferences tended to decrease, P <0.05, P <0.01; dexamethasone group compared withGuilongkechuanning group, P <0.05, there are differences; asthma rather high, mediumand low dose compared to high-dose group than in the low-dose group, P <0.05; asthmarather high dose group and lung tissue TGF-β1expression levels lower priorityCyclinD1comparison in the dexamethasone group and Guilongkechuanning group,statistically significant, P <0.05, P <0.01.3.1.2The comparison of Pingchuanning of EGFmRNA、 PDGFmRNA、p-ERK1/2mRNA: There is difference between the model group and the normal group，P<0.01; compared with the model group, the treatment of lung tissue EGFmRNA,PDGFmRNA, P-ERK1mRNA, P-ERK2mRNA could cut its expression values, P <0.05,P <0.01; asthma rather high, medium and Pingchuanning low dose compared toPingchuanning high-dose group than in the Pingchuanning low-dose group, P <0.05;asthma lung tissue rather Pingchuanning high dose group EGFmRNA, PDGFmRNA,P-ERK1mRNA, P-ERK2mRNA lower expression levels than dexamethasone group andGuilongkechuanning group, statistically significant, P <0.05, P <0.01.4conclusions: PingChuanFang can adjust the activation of the ERK pathway, down cold Asthma lung tissue TGF-β1, CyclinD1expression, reducing the expression levelsof EGFmRNA, PDGFmRNA, P-ERK1mRNA, P-ERK2mRNA, thus, reduce airwayhyperresponsiveness, inhibit cold asthmatic rats inflammatory cell infiltration, reduceinflammation, improve lung function, thereby delaying the treatment of asthma andairway remodeling.