| Objective:Anti gout granules is mainly composed of Clerodendranthus spicatus,Cortex Phellodendri,Chinese Starasmine Stem,Folium Eucommia and other herds.Based on the theory of TCM,Using the methods of animal experiment to study the related Pharmacodynamics experimental for the Gout Granuls and Clerodendranthus spicatus,Chinese Starasmine Stem,Folium Eucommia.The Purpose of this study was to provide scientific basis for the basic experiment.To studied the fingerprint by the modern important quality control analysis technology and the purpose of this study was to improve the quality control of anti gout granules.Methods:?1?According to the maximum tolerance of mice to study acute toxicity test.?2?To studied the abirritation of mices on the premise of taking rotundine as a positive drug by acetic acid twisting method.?3?According to the mouse ear swelling experiment caused by dimethylbenzene to study the anti-inflammatory effects.?4?To studied the diuretic effect by monitoring the urine in mice.?5?According to the method of mouse hyperuricemia induced by hypoxanthine to study serum uric acid level and urine uric acid level of mice.?6?According to the method of mouse hyperuricemia induced by Uricase Inhibito to observe the change of serum uric acid and liver Xanthine oxidase?XOD?cotent.?7?To study the fingerprint of the anti gout granules by HPLC.Results:?1?The acute toxicity test show that anti-gout granules origina extract has no significant toxicity,the maximum tolerance of mouse is 252g/kg,it is equivalent to 156 times of human clinical daily dose,and it is safe to use in the range of clinical.?2?The original prescription of anti gout granules and the three drugs in the prescription can reduce the writhing times of mice,and it has analgesic effect on mice pain?P<0.05,P<0.01?.?3?It also can inhibit the ear swelling by the xylene and foot swelling by the carrageenan in mice,so it has a good anti-inflammatory effect?P<0.05,P<0.01?.?4?It can promote the excretion of urine in mice and has a good diuretic effect?P<0.05,P<0.01?.?5?It also can reduce the serum uric acid level and uric acid level in hyperuricemia mice,and inhibit the XOD activity in mouse liver?P<0.05,P<0.01?.?6?The anti-gout granules HPLC fingerprint conditions:chromato-graphic column:Luna 5?m C18?2?100A?4.6×250mm?.Mobile phase:methanol-0.1% phosphate buffer.Elution Procedure: 0.01?14?22?24?25?30?33?38?43?50?60min.The volume fraction of methanol is5%?23%?31%?31%?36%?36%?37%?37%? 44%?51%?100%.Flow rate:0.8m L·min-1.Detection wavelength:330nm.Column temperature: 35?.Sample size:10?L.Through the fingerprint of anti gout granules by HPLC identified 10 common peaks.The fingerprints of anti-gout granules that determined by high HPLC determine 10 Common Peaks.The similarity of fingerprints of 10 batches of anti-gout granules is 0.976,0.996,0.975,0.969,0.976,0.997,0.97,0.997,0.998,0.983 respectively.Conclusion:?1?The anti-gout granules of the original extract has no obvious toxic reactions,and it is safe to use in the range of clinical.?2?The antigout granules,Clerodendranthus spicatus,Chinese Starasmine Stem,Folium Eucommia have an effect on analgesic,anti-inflammatory,diuretic,uric acid and also inhibited the role of xanthine oxidase.?3?The anti-gout granule fingerprints established by HPLC is simple,accurate and reproducible,andcan be used as one of the quality evaluation methods of anti-gout granules. |
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