A Preliminary Study On The Efficacy And Mechanism Of Moxibustion On Mammary Gland Hyperplasia In Rats

Objective:Mammary gland hyperplasia(Mammary gland hyperplasia,MGH)is a neither inflammation nor tumor proliferative breast disease,usually onset in middle-aged women,It ranks first in the incidence of all breast disease.The nature of mammary gland hyperplasia is a physiological hyperplasia and subinvolution caused normal breast structure disordered,breast lumps and pain as the main manifestations,and with the fluctuations of the menstrual cycle or cyclical changes in mood.At present,the pathogenesis of breast hyperplasia is not entirely clear,most scholars believe that pathogenesis of hormone-related disorders,the main treatment in Western medicine is oral hormone therapy and surgery,Hormone drugs have many side effects,the course is long,and may increase the body's endocrine disorders.Moxibustion is one of the traditional Chinese medicine external treatment,coordinating Yin and Yang,warmming Meridians and Collaterals,removing blood stasis and stagnation,replenish the Qi and many other effects.Moxibustion has been widely used in clinical breast hyperplasia,and achieved good therapeutic effect.This topic through moxibustion at Ganshu,Zusanli,Sanyinjiao treatment of breast hyperplasia model rats were observed moxibustion on the diameter of the nipple,breast tissue morphology of pathological changes in rat model of mammary gland hyperplasia,hypothalamic arcuate nucleus GnRH(Gonadotropin-releasing hormone,GnRH)positive neurons,pituitary luteinizing hormone(luteinizing hormone,LH)mRNA expression and serum estradiol(estradiol,E2),progesterone(progesterone,P)levels effects,observed moxibustion curative effect on experimental breast hyperplasia,and explore the mechanism of moxibustion treatment of breast hyperplasia,in order to provide experimental basis for the study of the mechanism of moxibustion treatment of breast hyperplasia.Methods: Female SD rats were randomly divided into normal group,model group and moxibustion group and grip group,15 in each group,The breast hyperplasia model of model group and moxibustion group prepared with intramuscular injection of estrogen and progesterone,normal group received no treatment,moxibustion group after a successful modeling moxibustion at Ganshu,Zusanli,Sanyinjiao,once a day,15 min once time;and gripping group with moxibustion group the same way as the gripping animals,but not moxibustion.duration 14 days,observation nipple diameter rats,HE staining of rat mammary gland morphology of pathological changes,adopted immunofluorescence staining in the hypothalamus arcuate nucleus GnRH positive cells,RT-PCR assay in rats pituitary LH mRNA expression,ELISA assay in rat serum E2,P levels.Results:(1)Compared with the normal group,model group significantly increased the diameter of the nipple,breast tissue significantly hyperplasia,after moxibustion treatment,moxibustion rats nipple diameter than the model group was significantly reduced(P <0.05),breast tissue greatly improved the situation,the nipple diameter of the normal group and gripping group did not change significantly;(2)compared with the normal group,model group rats hypothalamic arcuate nucleus GnRH positive cells increased significantly,compared with the model group,moxibustion rats hypothalamic arcuate nucleus significantly reduced GnRH positive cells;(3)compared with the normal group,the expression of rat pituitary LHmRNA model group increased significantly,compared with the model group,the expression of rat pituitary moxibustion group LHmRNA significantly reduced;(4)compared with the normal group,the content of serum E2 group rats significantly increased and serum P decreased significantly,compared with the model group,serum E2 level was significantly lower in rats moxibustion and serum P was significantly higher(all P <0.05).Conclusion: moxibustion therapy can significantly improve the model of rat mammary gland hyperplasia breast tissue morphology,reduced tumor size of breast hyperplasia,reduce hypothalamic arcuate nucleus Gn RH cells and pituitary LHmRNA positive expression,and can adjust the serum E2,P content,suggesting that moxibustion have good therapeutic effect for breast hyperplasia,suggesting that moxibustion mechanism may be under the control of GnRH secretion by hypothalamic arcuate nucleus to regulate the hypothalamus-pituitary-ovarian axis(hypothalamic-pituitary-ovarian axis,HPOA),thereby enabling the estrogen and progesterone levels return to normal achieve the purpose of treatment of breast hyperplasia.