| Atractylodis Macrocephalae Rhizoma is gained from rhizome of Atractylodes macrocephala Koidz, generality of which are cultivation.It's warm~natured, sweet flavor, attribution to the spleen and stomach.It has the effect of invigorating spleen and supplementing qi?drying dampness and diuresis ? strengthening exterior and reducing sweat. Atractylodes macrocephala Koidze is our traditional Chinese herbal medicines commonly used. It was classified as high grade in Shen Nong's Herbal. Its main varieties are cultivated in the market, the annual volume of 7 million kg.In the ancient herbal documents, northern and southern dynasties ago, it's not really discriminated between The Atractylodes macrocephala Koidze and The Atractylone but Tao Hongjing began distinguishing between them. Before the Tang Dynasty, there had a writing record for standards of commodity classes of Atractylodes macrocephala Koidze. In ancient times differentiating standards of commodity classes of Atractylodes macrocephala Koidze mainly depended on surface colour and the section of the oil of the Atractylodes macrocephala Koidze. Existing standards of commodity classes of Atractylodes macrocephala Koidze was depended on<Seventy~six kinds of medicinal products specifications>, which was enacted by State Medicine Administrative Bureau and ministry of health of China in 1984, this standard is mainly based on the size of Atractylodes macrocephala Koidze to divide the standards of commodity classes and Atractylodes macrocephala Koidze can be divided into four grades. However, the content of this standard, which ignored the connection between the traditional morphological indexes and the modern chemical index which associated with quality with the standards of commodity classes of Atractylodes macrocephala Koidze, has been disconnected with the present science and technology; do not accord with the actual market situation.Delphi method, also called expert investigation method, is based on the communication that experts in back to back to ask their predictions, is especially suitable for all kinds of experience of evaluation index system of inheritance and standardized research. At present has been widely applied in the field of traditional Chinese medicine(TCM) identification.Modern experimental studies had shown that atractylenolide class composition is an effective ingredient of Atractylodes macrocephala Koidze, also is the main characteristic of Atractylodes macrocephala Koidze. Especially atractylenolide I ? III had significant pharmacological activity and relatively stable chemical properties. Reference substance was more easily also. Its content can be used as the main index in the quality control of Atractylodes macrocephala Koidze.This study through the "Delphi method" to make the correlative analysis between characters of Atractylodes macrocephala Koidze and its standards of commodity classes, by high performance liquid chromatography(HPLC) to determine the content of atractylenolide I ? III which is the main effective components. Through the analysis of mathematical statistics, Authors establish the correlation between traditional character and modern chemical index and the standards of commodity classes of Atractylodes macrocephala Koidze. Ultimately conclude the standards of commodity classes of Atractylodes macrocephala Koidze which is meeting the requirements of scientific laws and suitable for market.Objective:Using high performance liquid chromatography determine the content of atractylenolide I ?III,the sample contained which is Collected from the main medicinal materials markets. Using the Delphi method analyze traditional character, using the method of mathematical statistics to find the correlation between traditional character and modern chemical index and the standards of commodity classes of Atractylodes macrocephala Koidze. Make clear the relationship between external properties?intrinsic chemical composition and standards of commodity classes, thus proposed the new draft standards of commodity classes of Atractylodes macrocephala Koidze, providing the reference theory basis for the new commodity classes of Atractylodes macrocephala Koidze.Methods:1 sample collection and content determination of atractylenolide I ?III:(1) the sample collection: collected forty batches of Atractylodes macrocephala Koidze, on major medicinal materials market(2) content determination: Using high performance liquid chromatography determine the content of atractylenolide I ?III.(3) Correlation analysis: using the statistical software SPSS19.0 to analyze the correlation between medicinal materials content and standards of commodity classes finally got its inner link.2 Based on "Delphi method" to extract the traditional identify traits of Atractylodes macrocephala Koidze:(1) determine the panel members : Members should be an expert, whose working time is not less than 20 years, working in aspect of identification of Chinese drugs, Traditional Chinese medicine(TCM) inspection, Chinese Materia medical Commoditology and other related industries, was familiar with Traditional Chinese medicine(TCM)'production links, circulation links, the use of link etc.(2) Formulation evaluation expert questionnaire: through literature, soliciting opinions from relevant professionals and experts, combined with actual market demand, formulate expert questionnaire. The questionnaire should list the rating rules and requirements of the related indicators in the table, to provide the reference for the experts.(3) "back~to~back" evaluation and experts concentrated rate: first of all, in requirements of the blinded evaluation, ever separate experts make two evaluations, after the two evaluations, gather the results and summarizing to notice each expert, finally by the experts discussion, determine the related parameters of sensory evaluation specification.(4) using of statistical software SPSS19.0 using the statistical software SPSS19.0 to analyze the correlation between character indexes and standards of commodity classes finally got its inner link.Results:1 the determination of atractylenolide I ?III: The content of sample's atractylenolide I ?III were 0.0574 mg/g ~ 1.4737 mg/g and 0.0556 mg/g ~ 1.0813 mg/g.2 "Delphi method" to extract the specifications of Atractylodes macrocephala Koidze: The specifications which greatly influenced standards of commodity classes of Atractylodes macrocephala Koidze were the number of the section oil drop, color of the section, texture of the section, texture of surface, gas and taste, etc.3 According to the results of mathematical statistics, when content of atractylenolide I ?III were higher the medicinal materials had good quality, and the higher level; The more the number of the section oil drop, the better the quality Atractylodes macrocephala Koidze had; the deeper transverse color, the better the quality; The deeper Surface color, the better the quality; The more clear section chrysanthemum lines, the better the quality; The more the Cross~sectional hard, number of honeycomb holes, the better the quality.Conclusion:Through the study, initially set the draft standards of commodity classes of Atractylodes macrocephala Koidze, as follows:... |
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