Studies On The Polysaccharides Of Atractylodes Lance

Atractylodes are the rhizome of Atractylodes lancea (Thunb.) DC. or Atractylodes chinensis (DC.) Koidz. Atractylodes were called Atractylodes lancea (Thunb.) DC. because of its mainly product Mount Mao in JiangSu. At present, the experts focus on the study of the volatile component of Atractylodes, while they little concentrate on the water-soluble component of Atractylodes. But the water-soluble component of Atractylodes which had been studied was identified to have some pharmacological action. So the dissertation systematically studied the extraction technology, isolation and purification, composition analysis, molecular weight and initial pharmacological action. It aims to find out a new active component and to develop the valuable resource of Atractylodes.The dissertation was first studied the extraction technology of Atractylodes polysaccharides. Single factor test and orthogonal experiment design method L₉ (3⁴) were applied to analysis the effects of the extraction time, extraction temperature , extraction times and ratio of water to sample on extraction rate of polysaccharides of Atractylodes lancea. The optimal extraction technology was that the polysaccharides were extracted with water 20 times, 80℃, four hours and twice extraction. The yield rate was 3.90%. The determination of Atractylodes polysaccharide was 15.33% by phenol-sulfuric acid method. This method is convenient, and the stability and reproducibility are good.The Atractylodes polysaccharides extracted by hot water and the residue extracted by NaOH solution, deproteinization by TCA, and DEAE-52 cellulose column chromatography, the Atractylodes polysaccharide was separated three parts, APBd-1, APBd-2, APBd-3 . Then they purified by Sephadex G-100 column chromatography, received APBs-1, APBs-2, APBs-3. They are single symmetrical peaks detected by HPLC. It concluded that the three component of Atractylodes polysaccharide are pure polysaccharide. The dissertation analysised the monosaccharide composition and the ratio of the Atractylodes polysaccharide by TLC and GC. The results were that there are three monosaccharide in APBs-1, unknown monosaccharide, Rha and Xyl, its ratio is 1.80:1, there are Rha., Ara. and Gal. in APBs-2, the ratio is 1.12:8.89:1. The APBs-3 mainly composed Ara. and an unknown monosaccharide. And determined the correction factor vis Gal are 0.698, 0.766, 0.543. The contents of three parts of Atractylodes are 60.24%, 71.62%, 48.87%.The assay firstly studied the characteristics of Atractylodes polysaccharide and determined its molecular by UV, IR, GC, HPLC. The results were that Atractylodes polysaccharide was brownish red colored powder, tasteless, water-soluble and undissolved alcohol, acetone, chloroform, et al. The molecular weight of APBs-1 is 9.08×10³ Da, APBs-2 is 3.33×10⁴Da, APBs-3 is 10.95×10⁵Da. They showed characteristic absorption peaks on UV and IR. And supposed Atractylodes polysaccharide was gluciamin on IR. It cannot see the 810 cm^-1 and 870 cm^-1 absorption peak, so it concluded that the Atractylodes does not have Man. It had the same result with GC.The dissertation initially approached pharmaco-activity of Atractylodes polysaccharide. It had scavenging-effect to hydroxyl radicals. And with the concentration increased, the scavenging capability strengthened, it indicated that the crude polysaccharide of Atractylodes had dose-effect relation scavenging capability and concentration, the clearance rate was 35% when the concentration was 4.8 mg/mL. It had not obviously increased when the concentration larger than 5mg/mL. The crude Atractylodes polysaccharide promoted the AGS cell at the low level. With the concentration increased to 60μg/mL, the polysaccharide had inhibitory action to AGS. When the concentration increased 320μg/mL, the inhibiting capacity was weaker. The crude polysaccharide had the better inhibitory action to U937 cell. With the concentration increased, the inhibiting capacity was increased.