| BackgroundAfter people did research on HIV for so many years, they found that the disease process of HIV infection canbe divided into three stages:acute infection period, asymptomatic period and AIDS period. In the former two periods, most infected people present no or only mild symptoms which will not poses a serious threat to life; in the third period, patients will coinfect with all kinds of opportunistic infections which will lead to death. Although there is no effective antiviral drugs can cure AIDS now, however, we can significantly prolong patients’ life and improve the quality of their lives by antiviral therapy, it is also a kind of widely used method at present. Infants infect HIV mainly by mother to child vertical transmission, but they can’t be determined infection status by the conventional serological test, because HIV exposed infants’ antibodies may be inherit from mother. This paper compared consistency of the two methods, which can test HIV-1 by nucleic acid qualitative. This is very important to determine the babies’ HIV infection status and early antiviral therapy as soon as possible.ObjectiveTo compare the consistency in Early Infant Diagnosis using DBS between In-house method and Abbott Real-Time HIV-1 Qualitative method.Methods1. Collect DBS 217 cases of HIV exposed infants from 2013.10 to 2014.1, which were sent by Sichuan, Chongqing, Jiangsu, Xinjiang Province and so on to National AIDS Reference Laboratory in China CDC. Then they were detected by two methods: In-house to detect HIV-1 DNA and Abbott Real-Time Qualitative Kit;2. Then we compared the consistency of the results and performed statistical analysis by Spss19.0 software.Results1. For the 217 infant DBS, results of In-house methods were 10 positive and 207 negative, while results of Abbott Real-Time Qualitative Kit were 9 positive and 208 negative. Only 3 of these DBS showed different results by these two methods;2. After analyzing the results, we could calculate its consistency rate was 98.6% (214/217) and Kappa index is 0.835, P<0.05, which showed an excellent consistency between these two methods.ConclusionIn-house EID method has an excellent accordance with commercial Abbott Real-Time HIV-1 Qualitative method by using DBS, which shows that they both can be used for EID in China and this can be a scientific basis for how to choose an suitable EID test methods.BackgroundAIDS patients often coinfect with other viral due to their low immunity, including hepatitis virus, herpes virus, adenovirus, etc. Although HHART improved the clinical outcome of HIV infected people greatly, but the coinfection is still a thorny problem for doctors and public health experts. These viruses may be transmitted by the same route as HIV, or might already exist in the immune injury hosts, it will endanger the life if they cannot be diagnosised timely, it is one of the causes leading to death among AIDS patients. The vaccination and antiviral treatment for HIV infected infants who coinfected with other virus may be different from adults due to their special physiological characteristics. At present, the HBV and HIV-1 HHV infection rate in exposed infants is unclear, to understand the infection rate in this population in our country, and provide data support for selecting appropriate treatment and preventive measures for them, we did this research.ObjectiveTo know HBV、HSV、EBV、VZV、CMV, HHV-6、HHV-7 and HHV-8 co-infection rate in HIV-1 exposed infant in China, and provide a reference to lower their death rate.Methods1.Collected HIV-1 exposed infant DBS which were sent to National AIDS Reference Laboratory in 2011.01-2014.05, their HIV infection status have been determined by HIV-1 DNA qualitative testing;2. Selected HIV positive 106 cases and HIV negative 107 cases by means of stratified random sampling, as HIV positive group and HIV negative group;3.In addition, In January of 2015, we collected HIV unexposed infant DBS 100 cases as control group in Fengtai of Beijing, named HIV unexposed group;4.Tested HBV and Human Herpes viruses DNA respectively in these three groups, calculated their infection rate respectively and analysed the difference between them.Results1. In HIV positive group, we tested DBS 106 cases, the HBV infection rate was 2.83% (3/106), Human Herpes viruses co-infection rate was 3.77% (4/106), and HIV /HBV /CMV co-infection rate was 0.94%(1/106);2. In HIV negative group, there was no HBV infection, Human Herpes viruses infection rate was 0.94%(1/107);3. In HIV unexposed group, there was no HBV or Human Herpes viruses infection;4. HIV/HBV co-infection: Did X2 test on HBV infection rate of these three groups, Z= 2.1081, P<0.05 (P=0.0350), which indicated that there was significant difference between these three groups, and, the infection rate was HIV positive group> HIV negative= unexposed group;5. HIV/HHV co-infection: Did X2 test on HHV infection rate of these three groups, Z= 2.1693, P< 0.05 (P=0.0301), which indicates that there was significant difference between these three groups, and, the infection rate was HIV positive group> HIV negative> unexposed group; OR (HIV positive group/HIV negative group)= 4.Conclusion1. The results show that HIV/HBV co-infection in China infant is 2.83%, which is lower than in China childrens’4.9% in other research. This indicates there are 2.07% infants infected HBV through horizontal transmission;2. The OR value of HHV OR shows that, in HIV-1 exposed infants, the infect risk of HIV positive group is 4 folders of HIV-1 negative group. |
PDF Full Text Request