Experimental Research Of Tou Nong San On Wound Healing Of Rat's Abscess

Objective:To investigate the promotion of TouNong San on wound healing after rat's perianal abscess surgery.Method:Thirty-six SD rats were randomly divided into experimental group, positive control group and the model group. After the preparation abscess wound model in rats, positive control group was given Ding Ban He Ji and conventional treatment,the experimental group was given TouNong San and conventional treatment,and model group was given NS and conventional treatment. After administration of rat model In3th,7th,14th days the wound size was measured and calculates the wound area. The pathological changes about fibroblast hyperplasia and vascular proliferation, inflammation, edema were observed after operation for perianal abscess and treated with drugs. The granulation tissue of wound was obtained on the3th,7th,14th day. Scale of neutrophilic granulocyte, serum tumor necrosis factor-a (TNF-a),interleukin6(IL-6) in the3groups were compared as well as their clinical manifestations by obtioning carotid artery blood and the general situation of rats.Results:1Wound area:The wound area comparison of experimental group and positive control group at day of3,7,14, P=0.03,0.04,0.04,P<0.05,have significant. The wound area comparison of experimental group and the model group at day of3,7,14,P=0.028,0.003,0.002,P<0.01, have significant(P<0.01), The wound area comparison of the control group and the model group at day of3,7,14, p>0.05.2Serology:TNF-a and IL-6targets is the highest in three days,TNF-a and IL-6were decreased in seven day, However, the experimental group decreased more significantly than the positive control group P=0.01,0.04(P<0.05).Two treatment groups of inflammatory cytokines on day14recovered to normal levels. And the positive control group are still significant differences (P <0.05).3Pathology:In the7th and14th day after the administration,the angiogenesis of experimental group and positive control group P=0.049,0.02,P<0.05, the difference was statistically significant; In the3th and7th day after the administration, the fibrosis of experimental group and positive control group P=0.02,P<0.05, the difference was statistically significant.Inthe area of inflammation and edema, P>0.05, the difference was not statistically significant.Conclusion:TouNong San can promote the growth of granulation tissue rat wound healing after surgery and can control of the wound and inflammatio.TouNong San can promote the healing of postoperative abscess. To some extent tong nong san can reduce TNF-a and IL-6both inflammatory cytokine levels quickly and effectively. All scattered provethat toun ong san promote healing in rats after wound abscess.May be associated with lower levels of inflammatory cytokines, inhibition of the inflammatory response,and promoting fibrous hyperplasia...