Study On The Regulation Mechanism Of BAIJIEZI Powder Effect On Airway Inflammation In Asthmatic Rats And Immunity

Objective : Study of ancient days moxibustion medicine semen Sinapis Albae scattered different ratio of asthmatic rat airway and lung tissue pathology change, explore the effects on airway inflammation in asthma. Study of ancient days moxibustion medicine white mustard powder three kinds of different proportion of asthma rat serum IgE, IL-4, IFN, IL-2, EOS, MBP effect and study in the treatment of asthma immune regulation mechanism; according to the experimental results determined ancient days medicinal moxibustion BAIJIEZI powder the best ratio, for further development and promotion of winter disease in summer Zhitian of moxibustion therapy in treating asthma and provide drug applicator.Methods:White mustard powder most common compatibility proportion as the research object, diffusion effect on asthmatic rat airway inflammation and immune function by three different compatibility proportion of white mustard seed, to observe pathological changes of asthmatic rat airway and lung tissue, and the observation of the IgE, the changes of eosinophil(EOS), IL-2, MBP eosinophil. The double anti sandwich enzyme linked immunosorbent assay(ELISA) determination of the content of serum IgE, IL-4, IFN, EOS to the use of modern science and technology, from the molecular level of white mustard scattered airway inflammation of asthmatic children effect and mechanism of immune regulation, clinical extended winter disease in summer Zhitian moxibustion therapy provide experimental basis.Results:(1) Compared with the blank control group, the serum IgE, EOS, IL-2, MBP values were significantly changed, and there were significant statistically significant(P<0.05). In the course of the experiment, the experimental rat appeared in the experiment, and the experimental asthma model was successful.(2) Three Chinese medicine paste deposited treatment group and model group compared. Numerical test has changed, the 3:1:1:1 matched group(low dose group) and the asthma model group compared to a number of values, IL-2, EOS, MBP value significantly change, there were extremely significant(P < 0.01). It shows that the application of TCM in the treatment of asthma is meaningful, and the low dose proportion is the most reasonable in clinical application.(3) Budesonide group, budesonide + middle dose group, the asthma model group showed no significant difference, no statistical significance test value. Description in the aerosol inhalation of budesonide western medicine does not significantly reduced airway inflammation, improve eosinophils eosinophil infiltration. Phase comparison, + budesonide dose group group the project detection P values close to 0.05, the group was more significant than budesonide group, indicating atomizing and stick apply white mustard powder than simply aerosol inhalation of budesonide inhibit airway inflammation and improve immune function play a prominent role.Conclusion:BAIJIEZI powder of low dose group can significantly reduce the asthma specific marker of eosinophil count, EOS value, IL-2 basophilic protein MBP, IL-4, P<0.05 and the high dose group, there was significant difference between the middle dose group, low dose group; in addition, can increase immune function(immunoglobulin IgA, IgG) value, P<0.05 and the high dose group, there was significant difference, middle dose group, showed that the mustard powder sticking therapy for asthma by reducing the chronic inflammation with nonspecific inflammatory cell counts of eosinophils, basophils protein as the representative of the cause, at the same time improve the immunoglobulin IgA, IgG immune function to achieve the purpose of treatment. According to the experimental results, selected the best drug ratio is low dose group.