The Effective Component Dissolution Law Research Of Traditional Chinese Medicial Chrysanthemum From Different Origins

Objective To determine the optimum decoction time of the traditional Chinese medicial Chrysanthemum from different origins by studying its dissolved content of the effective components and the change law with decocting time; To obtain the best steeping times by researching the extraction content of the active ingredients from the Chrysanthemum with soaking times variation; To ascertain content difference and distribution characteristics of trace elements in Chrysanthemum from different areas by detecting the elements content.Methods The samples of Chrysanthemum both separated decoctions and combined decoctions in Sangju-Yin were drawn at different decoction time by adopting the traditional water decocting method. Chlorogenic acid and baicalin were taken as index components. The high performance liquid chromatography(HPLC) were used to research the change rules of dissolved content of correlative effective components in the decoction with decocting time. Other samples were drawn at different steeping times by adopting the regular tea-making method. Similarly, chlorogenic acid and baicalin were taken as index components. HPLC were used to research the change rules of dissolved content of correlative effective components with soaking times. A microwave digestion method were used in treatment of Chrysanthemum samples to measure trace elements content by microwave digestion-inductively coupled plasma atomic emission spectroscopy (ICP-OES).Results When Chrysanthemum were decocted both separately and combinedly in Sangju-Yin, Hangzhou Chrysanthemum was boiled at18min, and Chuzhou chrysanthemum at15min, and Huai chrysanthemum at17min, and Huangshan Tribute Chrysanthemum at l0min, the total dissolving-out amount of chlorogenic acid increased and baicalin increased to the highest ponit, and then the total content of them didn’t appear to add up as the decocting time continued to increase. When the four kinds of Chrysanthemum were brewed for three times, the dissolution percentage of chlorogenic acid was66.10%in Huangshan Tribute Chrysanthemum and baicalin was66.82%compared with the relatively highest content of correlative effective components in the decoction; Chlorogenic acid was dissolved out by73.45%in Hangzhou White Chrysanthemum, baicalin by87.04%as well; The dissolution percentage of chlorogenic acid was91.69%in Huai chrysanthemum, baicalin was83.04%as well; Chlorogenic acid was dissolved out by69.40%in Chuzhou chrysanthemum, baicalin by83.04%as well. There was still a small amount of dissolution of the effective components in Chrysanthemum if they were brewed more than three times. The content of five kinds of harmful heavy metal elements of this4Chrysanthemum was not beyond the standard. Trace element content curves of Chrysanthemum from different origins had a certain similarity; Results of principal component analysis manifested that the element of Ba、Cd、Co、Cu、 Mn、Zn、Pb and Sr might be the characteristic inorganic elements.Conclusions The traditional Chinese medicial Chrysanthemum should be decocted for about15min as the total dissolving-out amount of the effective components reached to the relative high point; As a kind of scented tea, Chrysanthemum should be brewed no more than3times, by the time the dissolution amount of the effective components achieves65%-90%. The content and its distribution and the results of principal component analysis of trace elements can reflect the characteristic of Chrysanthemums from different origins; The chromatographic process and elementary analysis methods which this research established is available for qualitative and quantitative analysis of the effective components and trace elements of Chrysanthemums and other relative traditional Chinese medicine.