HPLC In The Determination Of Chlorogenic Acid And Baicalin Preparations

Traditional Chinese medicine is the treasure of Chinese nation, it is thousands of years of splendid culture crystallization. In today’s world" regression is natural" in upsurge, it is becoming more and more popular. Quality is the preparation of traditional Chinese medicine modernization quota, drug quality, must have strict quality control standards. At present, in the preparation of traditional Chinese medicine Pharmacopoeia quality standardization problem did not get settlement, due to the lack of quality standards of Chinese traditional medicine for basic research, caused by an inability to control medicine quality standard.Traditional Chinese medicine composition is very complicated, usually used to thin layer chromatography method because of its precision, accuracy, reproducibility and sensitivity is poor and can not meet the demand of modern traditional Chinese medicine analysis. High performance liquid chromatography is its stability, reliability, high efficiency become the study of traditional Chinese medicine is the most important analysis method.The topics to chlorogenic acid and baicalin as an example, using high-performance liquid chromatography study on oral liquid of compound Portland and chlorogenic acid in Qingrejiedu oral liquid and baicalin content determination method of optimum conditions.The study were investigated to establish optimum chromatographic conditions:the choice of mobile phase and the acidity, the ratio of gradient elution, detection wavelength, column, flow rate, and so on. HPLC method validation:linear relationship, the detection limit, determination of content of the sample, precision, accuracy, recovery, stability, etc. Detection methods in order to determine the scientific.To determination of chlorogenic acid and baicalin content, this study refers to the relevent informadtion, by optimizing the chromatographic conditions, using gradient elution. Experiments show that the method is simple, rapid, accurate, high sensitivity, good reproducibility and low cost, which can be used for quality control of traditional Chinese medicines.