| Objective:The main topic of Cellulite parties observe spleen dyslipidemia in type 2diabetic rats body weight, blood glucose, blood lipids, serum insulin and liver tissues of MCP-1, Effect of expression levels of NF-КB.Cellulite square investigate spleen dyslipidemia in type 2 diabetes prevention mechanism to provide a scientific basis for clinical use.Methods:Abnormal T2 DM rat model established in this study lipids, after rats were randomly divided into the mold will become the model control group, spleen Cellulite group, simvastatin group, spleen statin group.Detection of blood glucose, blood lipids, FINS, IR-related indicators, the use of the situation NF-КB expression of MCP-1 in liver tissue observed by immunohistochemical protein level.And the use of real-time PCR technology, in the case of liver tissue MCP-1 expression was observed from the genetic level.Results:Spleen Cellulite model rats before lowering blood glucose, lipids and FINS level,compared with model group were significantly statistically significant(P <0.01), can reduce the water intake of rats, reduced body weight, with the model group was statistically significant(P <0.01).2. The model group rat liver tissue MCP-1 protein expression :①immunohistochemistry results showed that: the liver cells of rats in the control group,the expression of MCP-1 area than its predecessor, the strength is weak. MCP-1expression in the model group was significantly increased, spleen Cellulite group was weak compared with the model group(P <0.01). ② Quantitative analysis showed that :Compared with the control group, model group, liver tissue MCP-1 in the mean optical density(MOD) was significantly higher than the normal group(P <0.01);compared with the model group, the spleen liver tissue Cellulite group of MCP-1 mean optical density(MOD) was significantly lower than the model group(P <0.01), ③ rat liver tissue expression of MCP-1 m RNA : Compared with the control group, the model MCP-1m RNA group in liver tissue was significantly higher than the normal group(P<0.01); Compared with the model group, spleen and liver tissue Cellulite party group MCP-1m RNA significantly lower than the model group(P <0.05).Conclusion:1.Spleen Cellulite side has hypoglycemic, lipid-lowering, weight loss improve insulin sensitivity and reduce levels of inflammatory factors, improving the role of insulin resistance.2.Elevated liver tissue in a rat model of group MCP-1 protein and gene expression levels, suggesting that MCP-1 may be T2 DM dyslipidemia liver inflammation important inflammatory cytokines; spleen and liver tissue Cellulite only reduce MCP model group 1 protein and gene expression levels, MCP-1 may be the targets of Chinese medicine.3.Rat liver model group, the expression was significantly increased NF-КB proved NF-КB inflammation may also be one of the important factors in type 2 diabetes dyslipidemia liver inflammation; spleen Cellulite only reduce rat liver NF protein-КB, NF-КB may also be the targets of another traditional Chinese medicine. |
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