Experimental Study Bushentiaojing Soup With Polycystic Ovary Syndrome, Insulin-like Growth Factor And Androgen Effects

Objective This topic based on the basic theory of TCM, through experimental research, observation ofkidney menstrual function soup on polycystic ovary syndrome model rat ovarian insulin-like growthfactor1(IGF1) expression and the influence of the influence of serum testosterone (T), explore themechanism of the kidney and regulate the menstrual function of soup treating polycystic ovary syndrome.Methods This topic selection of about70days of age70SPF female rats for research object, theadaptability of feeding3days, to observe the rat vaginal exfoliated cytology changes for10days, therewill be no build complete out10rats oestrous cycle, the actual participation in experimental rats for a totalof60, the60rats in a ratio of1:4were randomly divided into blank group(12) and experimental group(48),Make mould method given in experimental rats with Poretsky subcutaneous injections of insulin andhuman chorionic gonadotropin made model of polycystic ovary syndrome, blank group and12dailyvolume, such as physiological saline injection in rats. After the building, random take eight rats in theexperimental group, and vaginal exfoliated cells smear observation, androgen levels of detection, themicroscopic observation ovarian tissue changes; Blank group according to the method of randomsampling by contrast, only2to verify that the building was successful. After the success of the building,the experimental group rats were randomly divided into four groups: model control group (model group),kidney menstrual function decoction high dose group (hereinafter referred to as high dose group) andkidney menstrual function soup low dose group (hereinafter referred to as the lower dose group) andwestern medicine control group, each group of10. Treatment under the same feeding conditions, at thesame time, the normal group, model group of each rat daily according to20ml/kg of distilled water, highdose group according to the38.0g of the crude drug/kg clothes; Low dose group according to the19.0gof the crude drug/kg, positive control group according to0.42g/kg daily irrigation taie-35, both for21days in a row. After21days, to be put to death in the rat, immunohistochemical method to detect theexpression of IGF1in rats; No method to detect the serum T level.Results (1) the expression of IGF1in rats: the influence of the known model control group were higherthan in the rest of the group, with statistical significance (P <0.05); Model control group and high dosegroup, low dose group, positive control group have difference (P <0.05); High dose group and positivecontrol group had significant difference (P <0.05); Low dose compared with positive control group between no difference (P>0.05).(2) the influence on rat serum T: statistical processing in the rat serum Tlevel, according to the model control group significantly increased levels of testosterone (T), the treatmentgroup reduced levels of serum testosterone (T), especially in high dose treatment group reduced levels ofserum testosterone (T) the most obvious (P <0.05).Conclusions Rats kidney menstrual function soup can reduce the expression of insulin-like growthfactor1in ovarian, regulating the function of the rat hypothalamus-pituitary-ovarian axis stabilized,androgen synthesis channel, reduce the high androgen hematic disease, so as to promote the normaldevelopment of follicle and induce ovulation successfully.