Association Between Plasma ALD And KCNJ5Gene Polymorphisms In Primary Hypertensive Patients

Objective：To investigate the association between plasma aldosterone(ALD) and KCNJ5(rs2604204) gene polymorphisms in primary hypertensivepatients.Methods：The new diagnosis of229primary hypertensive patients withoutany antihypertensive agents had been selected from October2011to January2014in the Department of Cardiovascular Diseases of Fujian ProvincialHospital. Hypertension patients were divided into two groups depend onthe median of ALD (183.18ng/l)：high ALD group(n=114)and control group(n=115). Clinical data, serum biochemical，rennin-angiotensin-aldostero-ne, cortisol, ambulatory blood pressure and echocardiography data wererecorded. Polymerase chain reaction (PCR) was used to characterize KCNJ5(rs2604204) genotypes(A/C).Results:(1)The frequency of different genotypes of KCNJ5(rs2604204) were inaccordance with Hardy-Weinberg equilibrium in our study (P>0.05).(2)Clinical characteristics： Compared with control group, thetriglyceride(TG), uric acid (UA), insulin, insulin resistance index,renin, angiotensin I, angiotensin Ⅱ, cortisol,24hSBP, dSBP, SD (PD),SD(PS), left ventricular mass index(LVMI) were high in high ALDgroup(P<0.05). The gender, age, height, weight,BMI, waist circumference,total cholesterol (TC), low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C), fasting blood glucose(FBG), glycosylated hemoglobin A1c(HbA1c)，sodium，potassium，24hDBP，dDBP，nSBP, nDBP showed no significant difference between two groups. (3)Gene polymorphisms：Compared with control group，the distributionfrequency of AC and CC genotypes of KCNJ5gene were more in high ALD group(x2=10.93，P=0.004)，and C allelic frequency was also more in high ALDgroup (x2=9.78, P=0.002). There was no significant difference between ACand CC genotypes of two groups. Compared with hypertensive patients withAA genotype, patients with C allelic showed increased high ALD risk(x2=9.808，OR=2.36495%CI：1.380-4.049，P=0.002).(4)Analysis of the risk factors of high ALD: After adjusting the TG, UA,cortisol, insulin, insulin resistance index, renin, angiotensin I,angiotensin Ⅱ, cortisol, logistic regression analysis showed that KCNJ5genotype，angiotensin Ⅱ entered into the regression equation，comparedwith hypertensive patients with AA genotype, hypertensive patients withC allelic showed increased high ALD risk (OR=2.225,95%CI：1.239-3.997，P=0.007).Conclusion: The KCNJ5(rs2604204) gene C allelic might be a susceptibilityfactor to the high ALD in primary hypertensive patients.