The Study Of Angiotensin Converting Enzyme Gene Polymorphism Among Tibetan And Han Hypertensive Patients

Objective Tibetan who is 38% of the population lives in a comparatively closed condition in Qinghai province. Most of them always get married with the same nationalities, which lead to the lacking of genetic exchange with other people. This study makes use of the special genetic resource in our province, studying the relationship between Tibetan and Han ACE genetic polymorphism and the risk factors of hypertension by the technology of the classical polymerase chain reaction sequence specific primers. Observing the ACE genetic susceptibility to provide the evidence for Tibetan and Han hypertension defend, as well as the target dot for gene therapy. Methods 84 Tibetan hypertensive cases, 80 Tibetan healthy cases,116 Han hypertensive cases, 97 Han healthy cases were collected in this study. ACE gene insertion/deletion (I/D) polymorphisms was detected by polymerase chain reaction (PCR) method, the distribution of DD, DI, II gene frequency were observed, the correlation between ACE genetic polymorphism and risk factors of hypertension was analyzed.Results DI, II DD, genotype frequency of Tibetan hypertensive group were 8%, 54%, 38%; Tibetan control group were 11.1%,55.6%, 33.33%, differences between the two groups were not significant (P>0.05). DI, II DD, genotype frequency of Han hypertensive group were 1%,43%,38.9%; Han control group were 16.5%, 25%,58.5%, differences between the two groups were significant (P<0.05). Body mass index (BMI), total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL) and high-density lipoprotein (H-DL) all had significant differences (P < 0.5) between hypertensive groups and control groups. Blood pressure levels were significantly different between D allele's carriers and II alleles carriers in Han hypertensive group. Blood pressure levels were not significantly different between D allele's carriers and II allele's carriers in Tibetan hypertensive group.Conclusion ACE I/D polymorphism may be the gene cause hypertension of Han, rather than that of Tibetan. Additional research is needed to better understand the relationship between ACE gene and risky factors of hypertension. Moreover, multiple genes and environmental factors should be considered in the further study.