Study On The Relationship Between The Coagulation System-related Genes Polymorphism With Hypertensive Disorder Complicating Pregnancy

Objective:The purpose of our study was to analyze the prothrombin gene G20210A mutation ,the FactorⅤLeiden(G1691A) mutation and their association with hypertensive disorder complicating pregnancy and placentas of pregnant women .Methods:Tetra-primer amplification refractory mutation system-PCR analysis was conduct -ed to examina the prothrombin gene mutation(G20210A) and the Factor V Leiden gene mutation(G1691A) with 66 placenta of hypertensive disorder compliating preg -nancy patients and 69 normal preg -nancies.Screening for the prothrombin gene and the FactorⅤLeiden gene polymorphisms (G20210A and G1691A) was carried out by analysis of polymerase chain reaction–restriction fragment length polymorphism . Statistical significance was regarded as p<0.05.Results:1. In the research of prothrombin G20210A polymorphism:(1)We found that the constituent ratio of AA,GA genotype between the hypertensive disorder complicating pregnancy group and the normal group had statistical significance (χ2=7.24,P =0.0268). In addition, the A allele frequency was significantly higher in the hypertensive disorder complicating pregnancy group (49.2%) than that in the control group (33.3%), too. there were statistically significant differences for the frequency of A allele of prothrombin genes between the hypertensive disorder complicating pregnancy group and the normal group.(2)We used logistic regression models to estimate the odds ratios of risk for hypertensive disorder complicating pregnancy associated with prothrombin gene polymorphism compared with GG genotype,there was a significant relationship between AA genotype of prothrombin gene of hypertensive disorder complicating pregnancy (OR; 3.63,95% CI; 0.325～7.678) .The frequency of the A allele of the prothrombin polymorphism plus G allele was significantly higher in preeclamptic patients than in controls (OR=1.94, 95%CI:1.455～2.587). 2. In the research of coagulation factorⅤgene polymorphism(1)We found that the constituent ratio of AA,GA genotype between the hypertensive disorder complicating pregnancy group and the normal group had statistical significance (χ2 =12.38,P =0.0021). In addition, the A allele frequency was significantly higher in the hypertensive disorder complicating pregnancy group (53.0%) than that in the control group (31.2%), too. there were statistically significant differences for the frequency of A allele of coagulation factor V genes between the hypertensive disorder complicating pregnancy group and the normal group.(2)We used logistic regression models to estimate the odds ratios of risk for hypertensive disorder complicating pregnancy associated with coagulation factorⅤgene polymorphism compared with GG genotype,there was a significant relationship between AA genotype of coagulation factorⅤgene of hypertensive disorder complicating pregnancy (OR; 5.97,95% CI; 2.067～17.253) .The frequency of the A allele of the coagulation factorⅤgene polymorphism plus G allele was significantly higher in preeclamptic patients than in controls (OR=2.49, 95% CI;1.516～4.087).3. Combination of prothrombin G20210A gene and coagulation factorⅤG1691A gene polymorphismsWe used logistic regression models to estimate the odds ratios of risk for hypertensive disorder complicating pregnancy associated with prothrombin G20210A and coagulation factor V gene polymorphisms. compared with GG-GG genotype combined,there was a significant relationship between GA-AA,GA-GA,AA-GA and AA-AA genotype combined of hypertensive disorder complicating pregnancy . (OR;6.67,95% CI; 2.106～21.136, Fisher exact probability was 0.0007) .Conclusion:1.We conclude that the placentas's prothrombin gene G20210A polymorphism may have correlation to preeclampsia,the presence of the G20210A gene in placentas could be a marker of increased risk of developing preeclampsia.2. We conclude that the placentas's coagulation factor V gene G1691A polymorphism may have correlation to preeclampsia . the presence of the G1691A gene in placentas could be a marker of increased risk of developing preeclampsia. 3. Synergism of prothrombin G20210A and coagulation factor V polymorphisms of blood clotting system may be a risk factor for hypertensive disorder complicating pregnancy.