| AIM：By observing the expression of CD44mRNA in surface of peripheral bloodmononuclear cells(PBMC) and the concentration of interleukin-6(IL-6) in plasmain patients with different types of coronary heart diseases(CHD),to investigatethe relations of the expression of CD44mRNA in surface of PBMC and CHD andthe severity of the illness, as well as the correlation of it and the IL-6in plasma.Methods:130patients who have accepted coronary angiography (CAG) in FujianProvincial Hospital Cardiovascular Medicine were enrolled in this study.Collected6ml peripheral blood from all entrants in the fasting next morning andseparated plasma and peripheral blood mononuclear cells (PBMC). Theexpression levels of CD44mRNA in surface of PBMC were measured by realtime reversed transcript polymerase chain reaction(PT-PCR) after extracting totalRNA of PBMC. Measured the IL-6in plasma by enzyme-linked immunosorbentassay (ELISA). Compared the expression of CD44mRNA in surface of PBMCwith CHD and its condition, as well as with the IL-6in plasma.Result:1.Age, gender ratio, body mass index(BMI), systolic blood pressure(SBP),diastolic blood pressure(DBP),total cholesterol (TC),triglyceride(TG), highdensity lipoprotein-cholesterol(HDL-C), low density lipoprotein-cholesterol(LDL-C) and fasting blood glucose(FBG) among the control group, SAP group,UAP group, and AMI group, shew no significant difference(P＞0.05). Thehigh-sensitivity C-reactive protein(hsCRP) in plasma shew significant difference(P＜0.05) by comparing between any two groups of the control group, SAPgroup, UAP group, and AMI group.2.Coronary artery Gensini scorings of the AMI group and UAP group were both higher than the SAP group and there were both significant difference (P＜0.05).The difference of coronary artery Gensini scorings between the AMI group andUAP group was no statistical significance (P＞0.05).3.The expression of CD44mRNAin surface of PBMC between the SAP groupand was no statistical significance (P＞0.05),but the UAP group and AMI groupwere both higher than the SAP group and the control group (P＜0.05), also theAMI group than the UAP group (P＜0.05).4.The concentration of the IL-6in plasma increased successively among thecontrol group, SAP group, UAP group, and AMI group and the IL-6in plasmashew significant difference via pairwise comparison (P＜0.05).5.As the condition of CHD aggravating, the expression of CD44mRNA in surfaceof PBMC, the IL-6and hsCRP in plasma all increased. There was significantpositive correlation between the CD44mRNA in surface of PBMC and the IL-6as well as hsCRP in plasma(P＜0.05), also between the IL-6and the hsCRP inplasma(P＜0.05).6.The coronary artery Gensini scoring was positively correlated with theexpression of CD44mRNA in surface of PBMC, the IL-6and hsCRP in plasma inpatients with CHD.Conclusion:1.The expression of CD44mRNA in surface of PBMC in patients with CHDincreased significantly and it may be a sign of PBMC activation, involve in theprocess of atherosclerosis and be dangerous signal of unstable atheroscleroticdisease.2.The expression of CD44mRNA in surface of PBMC has a significant positivecorrelation with the coronary artery Gensini scoring in patients with CHD,therefore it can reveal the degree of coronary artery stenosis and may become aindex reflecting the severity of CHD.3.The expression of CD44mRNA in surface of PBMC has a significant positivecorrelation with the IL-6and hsCRP in plasma in patients with CHD. The IL-6and hsCRP in plasma both can up-regulate the expression of CD44mRNA in surface of PBMC. IL-6，hsCRP and CD44all can be used as inflammatorymarkers which predict atheromatous plaque instability of coronary artery. Thus,the further research of CD44signal conduction pathway can provide very goodclinical therapeutic targets for CHD. |
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