| Cynanchum bungei, Bai Should Wu in Chinese, is Asclepiadaceae plants. It is sweet, bitter, sexually tepid, with a nourishing liver and kidney, essence and blood, anti-aging effects such as Ukraine to refund. Cynanchum bungei are commonly used medicinal plants for Cynanchum auriculatum Royle ex Wight. Main produced in Jiangsu. authentic herbs for the property, mostly for cultivation. Cynanchum bungei containing C21 steroidal glycosides and other bio-active substances, and contains a variety of nutrients and bioactive substances, in recent years, studies have shown that C21 steroidal glycosides containing Cynanchum bungei and multi-active ingredients such as sugar, and rich in protein, amino acids and zinc, manganese, iron, calcium, potassium and other body essential trace elements, there are anti-cancer, anti-tumor, anti-aging, enhancing immunity and protection of liver functions.Commonly used traditional Chinese medicine "White Shouwu", its use has a long history, but not seen in the ancient Chinese Materia Medica in a separate listing of its records, but in the "fleece-flower root," name and use. Thinks there is Chibai of Ukraine in the first hours of its original plants of the other male and female, who hung red, white and female persons. The main sources of three kinds of Cynanchum auriculatum Royle ex Wight、Cynanchum bungei Decne、Cynanchum wilfoldii (Maxim.) Hemsl.Cynanchum auriculatum Royle ex Wight has been large-scale cultivation, at home and abroad have applied for the mainstream Baishouwu species, distributed in most provinces and autonomous regions, mainly produced in Shandong. Jiangsu (cultivation), Jiangsu Binhai County has cultivated more than 300 years history, has served as a local Medicinal and Edible use of the tonic.1 Question the meaning of legislationAs Cynanchum bungei contains necessary amino acid, trace elements, salts, vitamins and other bioactive nutrients, their role in nutrition and health care with ginseng, American ginseng comparable with good development prospects. It contains Baishouwu glycosides on the heart and liver protective effects of flavonoids contained in immune regulation, lipid-lowering and other effects, it is necessary to study the composition of Radix dialogue. in order to improve product development and formulation of products using quality standards to provide some scientific basis.2 Composition Determination(1) This paper studies the Cynanchum bungei ash. starch, protein and other ingredients in general method for the determination, through Cynanchum bungei tablets and powder Cynanchum bungei the determination of the general composition, understanding the basic components of Cynanchum bungei studied Cynanchum bungei of the impact of technology on samples of products for production and standard-setting to provide some methodological basis.Cynanchum bungei determination of total ash films mean 2.90%, SD±MD was 2.90±0.30%. Determination of ash powder Cynanchum bungei total mean of 0.59%. SD±MD was 0.59±0.05%.Determination of starch films Cynanchum bungei total mean of 60.3%, SD±MD was 60.3±2.2%. Determination of starch powder Cynanchum bungei the total mean of 80.0%, SD±MD was 80.0±4.4%.Determination of protein chip Cynanchum bungei the total mean of 0.107%, SD±MD was 0.107±0.003%. Determination of protein powder Cynanchum bungei overall mean of 0.109%. SD±MD was 0.109±0.005%.(2) This paper studies the Cynanchum bungei total flavonoids. saponins. polysaccharides and other biological method for determination of active ingredients to explore the biological active ingredients of total flavonoids and polysaccharides in the extraction process, through the orthogonal test to determine the most suitable parameters of the extraction process, in order to Cynanchum bungei of further research and development and utilization to provide theoretical basis.First, rutin complex as a standard sample using spectrophotometry Cynanchum bungei of total flavonoids. Determination of wavelength 510nm. the linear equation:A=3.3996x +0.0006667. r=0.9987. recovery rate 94.4%(n=6), precision of RSD=0.2%(n=5). With ethanol as organic solvent extraction of total flavonoids Cynanchum bungei of ethanol concentration, bath temperature and extraction time for orthogonal experiment, the results of Cynanchum bungei flavonoids extracted the greatest impact is the temperature of water bath, followed by ethanol concentration and extraction time, choose the best combination of orthogonal analysis of ethanol concentration of 70%. water bath temperature of 100℃. extraction time 2h. Cynanchum bungei Determination of Flavonoids in Tablets average value of 22.60mg/g.Second, the samples of total saponins is extracted, Amberlite-XAD-2 macroporous resin column pre-separation, in acidic conditions, vanillin and ginseng saponin generate colored compounds, in order to ginsenoside Re as a reference substance at 560nm than at Colorimetry. Linear equation:y= 0.0089x+0.0028, r= 0.9991, recovery rate 94.7% (n= 6), precision of RSD= 0.8%(n= 5). Cynanchum bungei chip determination of total saponins average 4.98mg/g.Finally, the phenol-sulfuric acid method of the polysaccharide samples were determined. Determination of wavelength 490nm. the linear equation:A= 0.0089x+0.0047, r= 0.9992, recovery of 100.3%(n= 6), precision of RSD= 0.4%(n= 5). Choice of materials-water ratio, extraction temperature, extraction time 3 as the study factor, depicting three levels, for L9(33) orthogonal experimental design to determine the optimal extraction of polysaccharide Cynanchum bungei process. Orthogonal experiment results showed that the extraction of polysaccharides in Cynanchum bungei the greatest impact is the extraction temperature, followed by feed-water ratio and extraction time.Orthogonal analysis selected the best combination of material-water ratio 1:20, extraction temperature 100℃, extraction time 2 hours. Cynanchum bungei polysaccharide films measured average 7.95mg/g. |
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