| [Objective] Characterize the suppressive effect of Qi Wei Li Fei Tang on a bronchial asthmatic Sprague-Dawley rat models in vivo. The evaluation were from aspects of pathology of lung tissue, inflammatory cell, cytokines and the expression of NF-kB in lung tissue. The mechanism of Qi Wei Li Fei Tang attenuating the inflammation of asthma model was further explained.[Method] Asthma model was established by10%OVA/A1(OH)3+0.0023%。 pertussis toxoid sensitization and1%OVA exposure.70SD rats were randomly divided into seven groups(Normal control group, model group, blocker group, dexamethasone group, Chinese medicine low-dosage group, middle-dosage group, high-dosage group).H&E staining pathological section of lung tissue was made to observer the pathological changes. Cells in BALF were stained by Wright’s stain the counts the eosinophilic number in BALF under the optical microscope. The concentration of IgE and cytokines in serum were measured by ELISA. Proportin of associated cytokine was determined based on ELSIA data. The expression of NF-κB/p65and IκBα protein in lung tissue was detected by immunohistochemistry (Envision).[Result](1) The rats generally observed:normal control rats breathing steady, no asthma symptoms. The remained rats in each group were different degrees of expiratory wheezing, sneezing, restless, cyanotic lips and other typical asthma-like symptoms.(2) EOS counts in BALF:Compared with the nomal control group,the result of the model control group’s BALF smear EOS count was significant elevated (P<0.01); the blocker control group’s was a little higher(P>0.05)no statistically significant; the Chinese medicine low-dosage, middle-dosage and high-dosage.groups’ were higher(P<0.01) and showed the concentration-response relationship;and the dexamethasone group ’s was higher too(P<0.01).Compared with the model group,the blocker group’s was lower (P<0.01);the Chinese medicine low-dosage,middle-dosage and high-dosage groups’ were lower too,but the low-dosage group’s P>0.05(no statistically significant),when the middle-dosage and high-dosage groups’ P<0.01;and the dexamethasone group ’s was lower(P<0.01).Compared with the blocker group,the Chinese medicine low-dosage,middle-dosage and high-dosage groups’ were higher(P<0.01);and the dexamethasone group ’s was higher too,but P>0.05(no statistically significant). Compared with the dexamethasone group, the Chinese medicine low-dosage, middle-dosage and high-dosage groups’ were higher, but the high-dosage group’s P>0.05(no statistically significant), and the low-dosage, middle-dosage groups’ P<0.01. the results of BALF smear EOS count from high to low among every group was the model control group, the Chinese medicine low-dosage,middle-dosage, high-dosage groups, the western medicine group, the blocker control group, the blank control group.(3) lung tissue HE stain:The nomal control group had not showed any change and inflammatory cellular infiltration, when the blocker control group had a little,and the other groups all had inflammatory cellular infiltration in the lung tissue, bronchial epithelial cells irregular arrangement and desquamation, thickened alveoli septum and bronchiolar spasm,but the pathological damage severity were different. Results of the score by using tissue injury and inflammation pathological scoring criteria.(4) The content of IgE in serum:Compared with the nomal control group, every group’s serum IgE content increased (P<0.01), and the Chinese medicine low-dosage, middle-dosage and high-dosage groups showed the concentration-response relationship. Compared with the model control group, the blocker control group’s was lower (P<0.01);the Chinese medicine low-dosage, middle-dosage and high-dosage groups’were lower too,but the low-dosage group’s P>0.05(no statistically significant),when the middle-dosage and high-dosage groups’ P<0.05; and the western medicine group’s was lower(P<0.01).Compared with the blocker control group,the Chinese medicine low-dosage, middle-dosage and high-dosage groups’were higher(the low-dosage group’s P<0.05,while the middle-dosage and high-dosage groups’ P<0.01);and the western medicine group’s was higher too, but P>0.05(no statistically significant). Compared with the western medicine group, the Chinese medicine low-dosage,middle-dosage and high-dosage groups’ were higher,but the high-dosage group’s P>0.05(no statistically significant), and the low-dosage group’s P<0.01,the middle-dosage group’s P<0.05.(5) the expression of NF-κB/p65and IkBα protein in lung tissue：compared with normal control group, every group’s NF-κB/p65expression were significantly elevated P<0.01. Compared with the model group,the nomal control group, blocker group, dexamethasone group and Chinese medicine high-dosage group’s NF-k B/p65expression were significantly reduced P<0.05. Chinese medicine middle-dosage group and Chinese medicine low-dosage group’s expression low too P>0.05with no statistically significant.The expression of IκBa protein in lung tissue between each groups have significant differences:IkBα protein expression highest in nomal control group,the blocker group’s expression has decreased P>0.05with no statistically significant.the other group’s decreased significant P<0.01. The model group is the lowest one, The expression in other group’s are higher significant P<0.01. compared with blocker group, Chinese medicine group’ s were lower and showed the concentration-response relationship; Chinese medicine high-dosage group’s decreased less P>0.05with no statistically significant.compared with dexamethasone group, the Chinese medicine low-dosage,middle-dosage and high-dosage groups’ were lower P <0.01.(6) Test results of serum cytokine levels:the content of proinflammatory cytokines (IL-1β、IL-2、IL-4、IL-5、IL-13、IL-17、TNF-a) were significant increased P<0.01, the content of inflammatory suppression cytokines(IL-10) significant decreased P<0.01.and the ratio of Thl/Th2、Treg/Th17significant decreased P<0.01with good regulation role of Thl/Th2imbalance.compared with the model group,the ratio of IL-10/IL-17in high-dosage and middle-dosage group were higher P<0.01,with no aignificant difference between dexamethasone group P>0.05, with good regulation role of Treg/Thl7imbalance.[Conclusion](1) Qi Wei Li Fei Tang has effective treatment for rats bronchial asthma model:Qi Wei Li Fei Tang has anti-inflammatory effects on Sprague-Dawley rat bronchial asthma model were evaluated from aspects of ethology, pathology of lung tissue, inflammatory cells and expression of NF-KB/p65and IκB in lung tissue.(2)Qi Wei Li Fei Tang plays a role in regulating expression level of NF-kB/IκBain lung tissue:High-dosage of Qi Wei Li Fei Tang could significantly inhibit the activation of the transcription factor of inflammatory.(3) Qi Wei Li Fei Tang plays a role in regulating serum cytokine:Qi Wei Li Fei Tang could decreased the content of proinflammatory cytokines(such as IL-1β、IL-2、TNF-α、IL-4、 IL-5、IL-13、IL-17).The high-dosage group could effectively regulate the secretion of cytokine to keep the balance of Treg/Thl7and Thl/Th2. |
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