Effects And Mechanisms Of Huangqi Injected In Zusanlin On Spontaneous Sweat Rats Induced By Qi Deficiency

Along with environment pollution as well as population old age, qi deficiency ismore and more common in the modern society crowd. Its patient displays for the symptomof spontaneous sweat. Huangqi is the traditional Chinese herb which can restores vitalenergy, in clinical, using Zusanli acupuncture points injections treatment to curespontaneous sweat obtain the extremely good effect..This article will carry on theappraisal on the animal model to its drug efficacy, and will carry on the preliminarydiscussion to its function mechanismPart one:Objective to establish the model of spontaneous sweat rats induced by qi deficiencyand offer a method to evaluate Huangqi’s function on sweat rats induced by qi deficiencyMethods: Made the rats model with double elements of smoking and douchingMahuang decoction.Observe the change of behavior, weight, eating and frequency ofbreathd;Count the quantity of sweat beads; Observe pathohistological change of lung andsweat glands of toes.Result:We can see the rats of modle weary, short of breath and its appetite and weightreduced. The coulor of its fur turn yellow.The pathohistological change of lung wassignificant.The number of sweat glands of toes increased.Part Two:Objective to investigate the mechanism of Huangqi injected in Zusanli on spontaneoussweat rats induced by qi deficiencyMethods: Take an injection of Huangqi at Zusanli on the spontaneous sweat ratsinduced by qi deficiency. Observe the change of weight, appetite. Count the quantity ofsweat beads. Using thermometer to measure the rats’ temperatures. Using instruments tomeasure cruor and the blood cells,.SOD MDA and NO were examined by reagent boxes. Measure IL-1、IL-2 by MTT, observe pathohistological change of lung and sweat glandsof toes. Using theometer to measure blood stream. Using spectrophotometer to measure thecontents of Ach and AchE. Using the method of Elassa to detect cAMP, PGE2 andBeta-Endorphin of. Hypothalamus.Result:The weight and the appetite of rats taking an injection of Huangqi at Zusanliincreased. Its’ temperatures and the quantities of sweat beads decreased.. It can improve theblood viscosity, increase the content of SOD and decrease WBC, MDA and NO.Thelevel of IL-1、IL-2 in BALF were significantly elevated. It can improve thepathohistological change of lung, decrease the number of sweat glands of toes, increaseblood stream.The counts of total Ach decreased and AchE increased. The counts of cAMPand PGE₂ of hypothalamus decreased.Part Three:Objective to investigate the mechanism of Huangqi on endodermits cells injuried byH₂O₂Methods: By setting up the oxidative injury model of endodermits cells, observe theprotection of blood serum sampled from different time. We observed the morphologicchange of endodermits cells injuried by H₂O₂ and the antioxidation of Huangqi by testingthe level of NO, SOD and MDA.Result:After one hour of injecting Huangqi at Zusanli, the function of the bloodserum got the climax.It can improve the daminification of cells’ morphologic change. Itshowed that Huangqi could reduce the release of NO and MDA, increase the level of SOD.