| Objective:To explore the therapeutic effects and possible mechanisms of QinBojiajianfang to ulcerative colitis by detecting the expression of nuclear factor(NF-κB p65) and apoptosis inhibitory protein (Bcl-2) in rats with ulcerative colitis.Methods:A total of48healthy male rats were randomly divided into four groups:the normal group, model group, OLZ group(western medicine group), QinBojiajianfang group(Chinese traditional medicine group),each group has12rats. In addition to the normal group,all of the rest three groups use an experimental rat model of ulcerative colitis which was established by giving trinitro-benzene-sulfonic acid(TNBS).Starting drug intervention after the model established successfully and the treatment will be continued for3weeks. Body weight、nature of stool and mental state of the rats will be observed during the duration.At the end of treatment,taking colon tissues on all groups for CMDI score, and then getting some slice for HE dyeing for histological score and the other parts for detecting NF-κB p65and Bcl-2using immunohistochemistry. Results:The expressions of NF-κB p65and Bcl-2were significantly different among the four groups(P<0.01). The expressions of NF-κB p65and Bcl-2were significantly increased in model group compared with the other groups(P<0.01). However, the expressions of NF-κB p65and Bcl-2were significantly decreased after treating with olsalazine (OLZ) and QinBojiajianfang (P<0.01), and there is no statistically differences between OLZ and QinBojiajianfang treatment group (P>0.05).Conclusion:The experimental results show that:NF-κB p65and Bcl-2in ulcerative colitis model rats were significantly higher expression; QinBojiajianfang with retention enema can effectively treat the rats with experimental ulcerative colitis, improves the general condition and alleviate the inflammatory damage of colon.These studies indicate that QinBojiajianfang can resist ulcerative colitis by suppressing the expression of NF-κB p65and Bcl-2. |
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