| OBJECTIVE:In this study we investigated the expression levels of stemness genesOct-4and SOX-2in cervical cancer to determine whether the proteinlevels of these two genes could be an important indicator of cervicalcancer prognosis.METHODS:（1）The expressions of Oct-4and SOX-2were detected byimmunohistochemical SP of method in52cases of cervical cancer and28cases of normal cervical tissue.（2）The expressions of Oct-4and SOX-2were detected by Q-PCR in12cases of cervical cancer and6cases ofnormal cervical tissue.RESULTS:（1）Result of immunohistochemical experiment.:①The expressionof Oct-4protein in cervical cancer tissues(46.1%,24/52) was significantlyhigher than that in normal cervical tissues(7.1%,2/28),(p<0.005);②the expression of SOX-2protein in cervical cancer tissues(59.6%,31/52)was significantly higher than that in normal cervical tissues(10.7%,3/28),(p<0.005).③Oct-4and SOX-2protein were significantly correlated withthe histological grader, but not correlated with the age, pathological type, FIGO of the patients(p＞0.05).④The expression of Oct-4was positivelyrelated to the expression of SOX-2in cervical cancer tissue(p<0.05).（2）Result of Q-PCR experiment:①SOX-2mRNA expression in cervicalcancer tissue（12.67+3.98） is higher than its expression in normalcervical tissue（1.5+0.548）（,t=9.53，p＜0.01）;Oct–4mRNA expressionin cervical cancer tissue（15.83+3.24） is higher than its expression innormal cervical tissue（1.333+0.516）,（t=15.11，p＜0.01）.③Nosignificant positive correlation between SOX-2mRNA and Oct-4mRNA.CONCLUSION:1. The expression levels of Oct-4protein or mRNA are both higherin cervical cancer tissue than in normal tissue.2. The expression levels of SOX-2protein or mRNA are both higherin cervical cancer tissue than in normal tissue.3. Oct-4and SOX-2expression may be an important indicator of theprognosis of cervical cancer. |
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