Screening Of Chitinase Forming Strains And Fermentation Conditions For Enzyme Production

Chitin, largest reserves after cellulose in the nature, is the second-largest naturalpolysaccharide. It is also the organic compounds containing the largest number of nitrogen onearth. Chitinase (Chitinase, EC3.2.1.14) is widely distributed in all biota from microorganismto higher plants and animals, and have a wide range of functions. Chitinase, which candegrade chitin into chitin oligosaccharides and simple sugars, has great potential value in thefield of environmental protection, medicine, food and basic life science research.The living environment of halobios is different from that of terrestrial life. All kinds ofmarine organism have special structure and function to maintain its life under this ecologicalenvironment. In this study, B-7was chosed to be the experimental strain and the effect ofmany factors on the ability of it to yield Chitinase was investigated. The optimumcomposition of the medium was obtained by orthogonal test. The results were as following:0.4%colloidal chitin,1.5%glucose,0.4%ammonium sulphate,0.1%yeast extract paste,40%seawater,60%distilled water. The optimal chitinase-producing contition was obtained by theresponse surface methodology (RSM) analysis. The optimum cultured condition was Nacl3.39concentration initial pH of medium6, inoculation5%,40mL/100mL of liquid volume,26℃,140r/min, and6days. Under this condition, it increased about5.4times compared with strainB-7.