An On-chip Integrated Pump Based On Osmosis Effect For Perfusion Cell Culture

Chip-based microfluidic system is generally accepted as an important part in micro total analysis systems (μTAS), which aims at performing various kinds of analytical processes by controlling the fluid in the microchannles. In any microfluidic system, the microfluidic driving is indispensable. In the present work, a non-mechanical micropump using osmosis effect was developed based on biosimulation method. Then, an improved osmotic micropump was integrated on a microfluidic chip, and the whole device was used for continuous perfusion cell culture and on-line immunofluorescent test.In chapter1, the development of microfluidic driving systems was reviewed. We focused on the present application of the pumps based on osmosis effect in the microfluidics. Moreover, the application of microfluidic systems on cell study was also described. At last, the consideration of our work design was given.In chapter2, a novel micro-pump based on osmosis effect was presented, and a microfluidic device integrated with an improved osmotic micropump was fabricated. The manufacturing process was introduced in detail.The factors affecting the flow rate and the performance of the micropump were primarily studied. The micropump featured power-free, small and portable. With100mg/mL PVP as driving agent, an osmotic area of0.98cm" and the room temperature of25～27℃, the average flowrate within50h was0.33μL/min, with a precision of4.3%(n=51). It was demonstrated that the micropump could provide stable flow-rate in working conditions of the common laboratory.In chapter3, the microfluidic chip integrated with osmostic micro-pump was used successfully for perfusion cell culture, and continuous refreshing of culture medium was achieved by the micropump. The important design considerations of the entire microfluidic perfusion culture system were discussed. CCL-187cells kept in good conditions during one week culture. Cell growth in microchannel was identified by an immunofluorescent test, in which all of the agents were driven by the integrated osmotic micro pump.In chapter4, the on-chip integrated osmotic micropump and the microfluidic cell perfusion culture system were summarized and predicted.